biostudies-arrayexpressManuela Garcia-CanovasSus scrofahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15241This study explores the biological role of interleukin-6 (IL-6) as a supplement during in vitro maturation (IVM) of porcine oocytes, aiming to enhance embryonic development and reduce apoptosis following in vitro fertilization (IVF). The objective was to determine whether IL-6 supplementation alters gene expression in day-7 in vitro-produced blastocysts. To achieve this, oocytes from prepubertal gilts were matured in IVM medium with or without 100 ng/mL IL-6, fertilized in vitro, and cultured for seven days. Viable blastocysts were collected, flash-frozen, and analyzed using the GeneChip® Porcine Genome Array to identify differentially expressed genes (DEGs). The results revealed significant upregulation of genes involved in lipid metabolism, cell proliferation, immune response, and embryo development in the IL-6 group, highlighting IL-6’s potential to improve oocyte developmental competence through modulation of key biological pathways.biostudies-arrayexpressGrowth Protocol - Culture of morulae was performed for 24 h in NCSU-23 medium supplemented with 0.3 mg/mL PVA and 100 ng/mL platelet factor-4 under a paraffin oil coverSample Treatment - The oocytes from prepubertal gilts were collected and matured in IVM medium either supplemented with 100 ng/mL IL-6 or without supplementationLabeling - The ds-DNA targets were purified, fragmented and terminally labeled.Sample Collection - Samples are a pool of 7 viable in vitro produced porcine blastocysts obtained from oocytes matured in vitro in the presence or abscense of interleukin 6.Scaning - Affymetrix Expression Command Console (Affymetrix.Intensity data from each GeneChip® array were normalized using the robust multiarray mean (RMA) method.Hybridization - A total of 4.5 µg of the fragmented and biotinylated ds-DNA was incorporated into a hybridization mix from a GeneChip Hybridization, Wash and Stain Kit (P/N 90720; Affymetrix).Nucleic Acid Extraction - RNeasy Micro Kit (P/N 74004; Qiagen Iberica, Madrid, Spain) was used to extract total RNA.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsData Transformation - The raw data were transformed using the log2 function and then normalize using quantile methods to derive individual intensity values.UnknownTranscriptomicsGenomicsProteomicstranscription profiling by arraySus scrofaExposure of in vitro maturing pig oocytes to exogenous interleukin-6 modulates transcriptome profiles of resulting blastocysts, upregulating developmental signaling pathwaysManuela Garcia-CanovasManuela Garcia-Canovas, Adelina Lopez-Jara, Inmaculada Parrilla, Alejandro Gonzalez-Plaza, Heriberto Rodriguez-Martinez, Maria A. Gil, Cristina Cuello, Emilio A. MartinezfalseTranscriptome profile of in vitro produced blastocysts derived from in vitro matured oocytes exposed to exogenous interleukin-6This study explores the biological role of interleukin-6 (IL-6) as a supplement during in vitro maturation (IVM) of porcine oocytes, aiming to enhance embryonic development and reduce apoptosis following in vitro fertilization (IVF). The objective was to determine whether IL-6 supplementation alters gene expression in day-7 in vitro-produced blastocysts. To achieve this, oocytes from prepubertal gilts were matured in IVM medium with or without 100 ng/mL IL-6, fertilized in vitro, and cultured for seven days. Viable blastocysts were collected, flash-frozen, and analyzed using the GeneChip® Porcine Genome Array to identify differentially expressed genes (DEGs). The results revealed significant upregulation of genes involved in lipid metabolism, cell proliferation, immune response, and embryo development in the IL-6 group, highlighting IL-6’s potential to improve oocyte developmental competence through modulation of key biological pathways.2025-06-30T00:00:00Z2025-06-18T15:26:31.766Z2025-06-18T15:26:31.766ZE-MTAB-15241EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0003789EFO_0005518EFO_0003816EFO_0003815EFO_0003969