biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsQi PanN/ATRIzolOptimal Dual-mode mRNA Library Prep KitBGISEQ-500RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15255HFpEF mouse model was established by a \"double hit\" of L-NAME and high-fat diet (60% fat). 5-week-old C57BL/6N mice were given the above intervention for 5 weeks (Early HFpEF group) or 12 weeks (Advanced HFpEF group). 17-week-old mice fed a normal diet were used as the Control group. After adequate perfusion, the hearts were harvested. Ventricular RNA was extracted for RNA sequencing.biostudies-arrayexpressSequencing - Using the BGISEQ platform (BGI-Shenzhen, China).Sample Collection - After anesthesia with tribromoethanol, the ventricle was obtained after systemic perfusion with PBS. Tissues of ~20mg were harvested and stored at -80°C.Nucleic Acid Extraction - Using the regular TRIzol method.Library Construction - Library was prepared using Optimal Dual-mode mRNA Library Prep Kit (BGI-Shenzhen). Library preparation is performed using Optimal Dual-mode mRNA Library Prep Kit (BGI-Shenzhen, China). A certain amount of RNA are denatured at suitable temperature to open the secondary structure, and mRNA is enriched by oligo (dT) attached magnetic beads. After reacting at a suitable temperature for a fixed time period, RNAs are fragmented with fragmentation reagents. Then First-strand cDNA is generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. The synthesized double strand cDNA is subject to end repairment reaction. After cDNA end repairment, a single ‘A’ nucleotide is added to the 3’ ends of the blunt fragments through A tailing reaction. Then the reaction system for adaptor ligation configured to ligate adaptors with the cDNAs, and finally, the library products are amplified through PCR reaction and subjected to quality control. Next, the single-stranded library products are produced via denaturation. The reaction system for circularization is set up to get the single-stranded cyclized DNA products. Any uncyclized single stranded linear DNA molecules will be digested.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesQi PanfalseRNA-Seq of hearts of control, early stage and advanced HFpEF miceHFpEF mouse model was established by a \"double hit\" of L-NAME and high-fat diet (60% fat). 5-week-old C57BL/6N mice were given the above intervention for 5 weeks (Early HFpEF group) or 12 weeks (Advanced HFpEF group). 17-week-old mice fed a normal diet were used as the Control group. After adequate perfusion, the hearts were harvested. Ventricular RNA was extracted for RNA sequencing.2025-12-31T00:00:00Z2025-12-31T02:02:47.729Z2025-06-25T13:30:59.521ZE-MTAB-15255ERP174024EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184