{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Seung Hee Lee"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15275"],"description":["To determine whether MsrB2 expression in the hypertensive heart is related to cardiovascular complications, WT and MsrB2 KO mice were infused with Angiotensin II for 2 weeks. After hypertensive induction, mice from each group were sacrificed, and the whole heart was extracted and used to perform RNA-seq."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - They were quantified with the KAPA library quantification kit (Kapa Biosystems, MA, USA) according to the manufacturer’s library quantification protocol. Following cluster amplification of denatured templates, sequencing was progressed as paired-end (2×150bp) using Illumina Novaseq 6000 (Illumina, CA, USA). performed by Bioneer in south korea.","Sample Collection - The libraries were prepared for 150bp paired-end sequencing using TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero H/M/R.","Library Construction - The fragmented mRNAs were synthesized as single-stranded cDNAs through random hexamer priming. By applying this as a template for second strand synthesis, double-stranded cDNA was prepared. After sequential process of end repair, A-tailing and adapter ligation, cDNA libraries were amplified with PCR (Polymerase Chain Reaction). Quality of these cDNA libraries was evaluated with the Agilent 2100 BioAnalyzer (Agilent, CA, USA). performed by Bioneer in south korea.","Nucleic Acid Extraction - mRNA molecules were purified and fragmented from 2μg of total RNA using oligo (dT) magnetic beads."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Seung Hee Lee"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of mouse hearts infused with Angiotensin II","description":"To determine whether MsrB2 expression in the hypertensive heart is related to cardiovascular complications, WT and MsrB2 KO mice were infused with Angiotensin II for 2 weeks. After hypertensive induction, mice from each group were sacrificed, and the whole heart was extracted and used to perform RNA-seq.","dates":{"release":"2026-03-01T00:00:00Z","modification":"2026-03-01T02:02:48.846Z","creation":"2025-06-25T21:00:03.924Z"},"accession":"E-MTAB-15275","cross_references":{"ENA":["ERP174051"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}