{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Markus Riessland"],"instrument_platform":["N/A","Illumina NovaSeq X"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15277"],"description":["FAM49B is a genetic risk factor for Parkinson's disease and plays an important role in immune cells. Our data indicates that FAM49B is specifically expressed in nigral microglia and downregulated with age."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - human microglia cell line HMC3 was used. After the completion of CRISPR/Cas9 KO of FAM49B,  total RNA was isolated from cell culture","Nucleic Acid Extraction - To isolate and purify the RNA, RNeasy plus (mini), Qiagen kit was used according to the manufacturer's protocol.","Library Construction - The library preparation was performed by using an Illumina seq library kit (performed by Azenta)","Sequencing - Illumina seq, 150bp, ~350M PE reads (performed by Azenta)"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Markus Riessland"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq experiment of human microglia cell line comparing wild-type control to FAM49B-knockout","description":"FAM49B is a genetic risk factor for Parkinson's disease and plays an important role in immune cells. Our data indicates that FAM49B is specifically expressed in nigral microglia and downregulated with age.","dates":{"release":"2026-01-14T00:00:00Z","modification":"2026-01-14T02:02:26.581Z","creation":"2025-06-25T21:15:18.726Z"},"accession":"E-MTAB-15277","cross_references":{"ENA":["ERP174053"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}