biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMarkus RiesslandN/AIllumina NovaSeq XRNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15277FAM49B is a genetic risk factor for Parkinson's disease and plays an important role in immune cells. Our data indicates that FAM49B is specifically expressed in nigral microglia and downregulated with age.biostudies-arrayexpressSample Collection - human microglia cell line HMC3 was used. After the completion of CRISPR/Cas9 KO of FAM49B, total RNA was isolated from cell cultureNucleic Acid Extraction - To isolate and purify the RNA, RNeasy plus (mini), Qiagen kit was used according to the manufacturer's protocol.Library Construction - The library preparation was performed by using an Illumina seq library kit (performed by Azenta)Sequencing - Illumina seq, 150bp, ~350M PE reads (performed by Azenta)OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMarkus RiesslandfalseRNA-seq experiment of human microglia cell line comparing wild-type control to FAM49B-knockoutFAM49B is a genetic risk factor for Parkinson's disease and plays an important role in immune cells. Our data indicates that FAM49B is specifically expressed in nigral microglia and downregulated with age.2026-01-14T00:00:00Z2026-01-14T02:02:26.581Z2025-06-25T21:15:18.726ZE-MTAB-15277ERP174053EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184