biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsPatrick NylundIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15278In-depth RNA expression analysis of four human derived breast cancer cell lines and their brain metastatic counterpart.biostudies-arrayexpressSequencing - Sequenced on a NovaSeq6000 S1-200 flow cell using the 100nt PE formatSample Collection - Cell lines are cultured in complete DMEM media containing 10% FBS and 1% Pen-Strep.Library Construction - Libraries were prepared using the KAPA Stranded mRNA-Seq KitNucleic Acid Extraction - Total RNA samples were isolated using the Maxwell® RSC simplyRNA Cells KitOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesPatrick NylundJason HusefalseRNA-seq of matched breast cancer and brain metastasis cell linesIn-depth RNA expression analysis of four human derived breast cancer cell lines and their brain metastatic counterpart.2025-08-22T00:00:00Z2025-08-22T15:32:14.516Z2025-06-25T21:19:26.872ZE-MTAB-15278ERP174054EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184