biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsYang Yangtranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15287From January 1, 2018 to December 31, 2018, there were 19 patients (from four hemodialysis centers) undergoing failing arteriovenous fistula (AVF) that were resistant to endovascular therapy with extensive fistula aneurysmal change (n=4), diffuse stenosis (n=13) or poor skin condition of the forearm (n=2). While 13 of them manifested good size and quality of the outflow vein (>10 cm long, >6 mm in diameter and <6 mm under the skin, BF >600 ml/min) to create of a new AVF upstream. They have been provided with an end-to-side anastomosis of the outflow vein with the more proximal inflow artery, together with ligation of the failing downstream AVF. During the operation, the small segment adjacent to the distal healthy access was exercised for the control access (CA) sample. Until December 31, 2019, 5 of 13 patients had suffered failing AVF again, followed by the excision of segment of high-grade stenosis for the failing access (FA) samples.biostudies-arrayexpressLabeling - Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, One-Color (Cat.# 5190-2305, Agilent technologies). Labeled cRNA were purified by RNeasy mini kit (Cat.# 74106, QIAGEN, GmBH, Germany). Each slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies) in Hybridization Oven (Cat.# G2545A, Agilent technologies).Scaning - Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies) with default settings, Dye channel: Green, Scan resolution=3μm, PMT 100%, 20bit.Nucleic Acid Extraction - Total RNA was extracted and purified using RNeasy micro kit(Cat#74004, QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).Hybridization - After 17 hours hybridization, slides were washed in staining dishes (Cat.# 121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat.# 5188-5327, Agilent technologies).Sample Collection - Following surgical removal of connective tissues of the AVF access, aseptically add tissue specimen to the tube.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsYang YangData Transformation - To begin with, the raw data was normalized with the quantile algorithm. The probes that at least 100% of the values in any 1 out of all conditions have flags in \"Detected\" were chosen for further data analysis. Differentially expressed genes were then identified through fold change as well as P value calculated with t-test. The threshold set for up- and down-regulated genes was a fold change>= 2.0 and a P value<= 0.05.falseGenes differently expression between human failed arteriovenous fistula (AVF) and the controlFrom January 1, 2018 to December 31, 2018, there were 19 patients (from four hemodialysis centers) undergoing failing arteriovenous fistula (AVF) that were resistant to endovascular therapy with extensive fistula aneurysmal change (n=4), diffuse stenosis (n=13) or poor skin condition of the forearm (n=2). While 13 of them manifested good size and quality of the outflow vein (>10 cm long, >6 mm in diameter and <6 mm under the skin, BF >600 ml/min) to create of a new AVF upstream. They have been provided with an end-to-side anastomosis of the outflow vein with the more proximal inflow artery, together with ligation of the failing downstream AVF. During the operation, the small segment adjacent to the distal healthy access was exercised for the control access (CA) sample. Until December 31, 2019, 5 of 13 patients had suffered failing AVF again, followed by the excision of segment of high-grade stenosis for the failing access (FA) samples.2025-07-21T00:00:00Z2025-06-30T11:50:17.077Z2025-06-30T11:20:02.404ZE-MTAB-15287EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815