biostudies-arrayexpressMagdalena Wójcik-JagłaHordeum vulgarehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15311Global warming may cause overwintering plants to lose tolerance to low temperatures owing to deacclimation caused by winter temperature fluctuations. This dataset presents the results of a research which aimed to find out how mutation in the HvBRI1 and HvCPD genes affects the signalling and synthesis of brassinosteroids, and in consequence, influences de-acclimation tolerance of barley. We verified a hypothesis that temperature-dependent changes in the expression of selected genes contribute to de-acclimation tolerance. This study was aimed to identify the mechanisms responsible for differences between mutants and their reference cultivar – Bowman. We investigated barley de-acclimation tolerance using two mutant lines: BW084, harboring a mutation in the HvCPD gene, and BW312, harboring a mutation in the HvBRI1 gene.biostudies-arrayexpressGrowth Protocol - Plants were sown in pots (30 cm wide and 15 cm long) and grown for 3 weeks in a greenhouse at 18 °C (day/night) in daylight (the experiment was conducted in November in Krakow, Poland, 50°03’ N, 19°55’ E). Next, the plants were placed in a growth chamber for cold acclimation at a temperature of 2 °C/2 °C (day/night), with a photoperiod of 8 h/16 h (day/night) and 250 umol m-2 s-1 PPFD for 3 weeks.Library Construction - mRNA was isolated using oligo(dT)-attached magnetic beads, and cDNA libraries were constructed, converted into single-stranded circular DNA (ssCir DNA)Sequencing - The RNA samples were sequenced in paired-end 100 bp mode on the DNBSEQ platform (BGI, Cambridge, MA, USA) with sequencing depth 40M in total.Sample Collection - Leaves from each mutant and Bowman cultivar were sampled after cold acclimation (CA) and after 24 h after de-acclimation (DA) in three biological replicates (leaves from three different plants). The leaves were immediately frozen in liquid nitrogen after sampling and stored at −80°C until use. Each sample was collected from the middle part of the youngest fully developed leaf and weighted 0.03–0.05 g.Sample Treatment - Half of plants were subjected to cold acclimation only (without de-acclimation) and sampled after beeing kept for 3 weeks in hardening conditions. They served as control for the experiment.Sample Treatment - After cold acclimation, half of plants was de-acclimated at 12 °C/10 °C (day/night) 8 h/16 h (day/night) and 250 umol m-2 s-1 PPFD for 24 hours.Nucleic Acid Extraction - Total RNA was isolated from 18 leaf samples using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany).OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - The TPM (Transcript Per Million) data transformation was used according to Zhao et al. (2021), J. Transl. Med. 19, 269.UnknownTranscriptomicsGenomicsProteomicsBGISEQ-500RNA-seq of coding RNAHordeum vulgareMagdalena Wójcik-JagłafalseBrassinosteroids Enhance Barley De-Acclimation Tolerance via Modulation of Chloroplast- Associated Gene ExpressionGlobal warming may cause overwintering plants to lose tolerance to low temperatures owing to deacclimation caused by winter temperature fluctuations. This dataset presents the results of a research which aimed to find out how mutation in the HvBRI1 and HvCPD genes affects the signalling and synthesis of brassinosteroids, and in consequence, influences de-acclimation tolerance of barley. We verified a hypothesis that temperature-dependent changes in the expression of selected genes contribute to de-acclimation tolerance. This study was aimed to identify the mechanisms responsible for differences between mutants and their reference cultivar – Bowman. We investigated barley de-acclimation tolerance using two mutant lines: BW084, harboring a mutation in the HvCPD gene, and BW312, harboring a mutation in the HvBRI1 gene.2025-07-17T00:00:00Z2025-07-03T16:58:05.587Z2025-07-03T16:58:05.587ZE-MTAB-15311ERP174611EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969