{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Agnieszka Strzałka"],"instrument_platform":["Illumina HiSeq 2500"],"study_type":["ChIP-seq"],"organism":["Streptomyces venezuelae"],"species":["Streptomyces venezuelae"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15314"],"description":["We studied the binding of AdpA protein to Streptomyces venezuelae chromosome during development (12 and 20 hour of growth) using AdpA-FLAG protein."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - A corresponding “input” DNA was prepared for each immunoprecipitation sample by taking a small fraction of the sonicated sample, followed by DNA precipitation.","Library Construction - For all samples, the purified DNAs were subject to library preparation and Illumina sequencing at the Laboratory of Molecular Neurobiology Centre in Nencki Institute of Experimental Biology","Sequencing - For all samples, the purified DNAs were subject to library preparation and Illumina sequencing at the Laboratory of Molecular Neurobiology Centre in Nencki Institute of Experimental Biology","Sample Collection - The liquid MYM medium supplemented with trace elements was inoculated to the final OD of 0.001 with the spores of S. venezuelae strains (Sven_WT – wild-type, control sample, and Sven_ΔadpA/adpA-FLAG – adpA deletion mutant complemented with adpA-FLAG under adpA native promoter) (Płachetka et al., 2021), and incubated for 12 and 20 hours at 30C with shaking (220 rpm). The cultures were prepared in two independent repetitions per strain using standard 250-ml flasks (flat bottom, w/o baffles) containing 50 ml of the medium."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Agnieszka Strzałka","Marcin Wolański"],"additional_accession":[]},"is_claimable":false,"name":"ChIP-seq experiment of AdpA binding to Streptomyces venezuelae chromosome","description":"We studied the binding of AdpA protein to Streptomyces venezuelae chromosome during development (12 and 20 hour of growth) using AdpA-FLAG protein.","dates":{"release":"2026-05-01T00:00:00Z","modification":"2026-05-01T01:03:24.001Z","creation":"2025-07-03T17:08:49.254Z"},"accession":"E-MTAB-15314","cross_references":{"ENA":["ERP174614"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002692","EFO_0005518","EFO_0004184"]}}