biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsAgnieszka StrzałkaIllumina HiSeq 2500ChIP-seqStreptomyces venezuelaeStreptomyces venezuelaehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15314We studied the binding of AdpA protein to Streptomyces venezuelae chromosome during development (12 and 20 hour of growth) using AdpA-FLAG protein.biostudies-arrayexpressNucleic Acid Extraction - A corresponding “input” DNA was prepared for each immunoprecipitation sample by taking a small fraction of the sonicated sample, followed by DNA precipitation.Library Construction - For all samples, the purified DNAs were subject to library preparation and Illumina sequencing at the Laboratory of Molecular Neurobiology Centre in Nencki Institute of Experimental BiologySequencing - For all samples, the purified DNAs were subject to library preparation and Illumina sequencing at the Laboratory of Molecular Neurobiology Centre in Nencki Institute of Experimental BiologySample Collection - The liquid MYM medium supplemented with trace elements was inoculated to the final OD of 0.001 with the spores of S. venezuelae strains (Sven_WT – wild-type, control sample, and Sven_ΔadpA/adpA-FLAG – adpA deletion mutant complemented with adpA-FLAG under adpA native promoter) (Płachetka et al., 2021), and incubated for 12 and 20 hours at 30C with shaking (220 rpm). The cultures were prepared in two independent repetitions per strain using standard 250-ml flasks (flat bottom, w/o baffles) containing 50 ml of the medium.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesAgnieszka StrzałkaMarcin WolańskifalseChIP-seq experiment of AdpA binding to Streptomyces venezuelae chromosomeWe studied the binding of AdpA protein to Streptomyces venezuelae chromosome during development (12 and 20 hour of growth) using AdpA-FLAG protein.2026-05-01T00:00:00Z2026-05-01T01:03:24.001Z2025-07-03T17:08:49.254ZE-MTAB-15314ERP174614EFO_0002944EFO_0004170EFO_0002692EFO_0005518EFO_0004184