{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Agnieszka Strzałka"],"organism":["Streptomyces venezuelae"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15315"],"description":["AdpA is transcription regulator and controls gene expression. The goal of RNA-seq experiment was to determine AdpA regulonsduring growth in liquid medium. Dataset contains two strains (adpA deletion mutants and wild type), two time points (12 and 20h)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - the liquid MYM medium supplemented with trace elements was inoculated to the final OD of 0.001 with the spores of S. venezuelae strains  and incubated for 12 and 20 hours at 30C with shaking (220 rpm). The cultures were prepared in two independent repetitions per strain using standard 250-ml flasks (flat bottom, w/o baffles) containing 50 ml of the medium.","Library Construction - Library preparation was performed by Genewiz (Germany).","Sequencing - RNA-sequencing was performed by Genewiz (Germany).","Nucleic Acid Extraction - At the time of collection (12 and 20 hours) the culture suspensions were mixed with the stop solution and immediately spun down. After discarding the supernatants the cell pellets were stored at -80C for further use."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"data_protocol":["Sequence Alignment - RNA-seq reads were mapped to S. venezuelae genome using bowtie2 (version 2.5.2) and processed with samtools (version 1.19.2)"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina HiSeq 2000"],"study_type":["RNA-seq of coding RNA"],"species":["Streptomyces venezuelae"],"pubmed_authors":["Agnieszka Strzałka","Marcin Wolański"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of Streptomyces venezuelae adpA deletion strain","description":"AdpA is transcription regulator and controls gene expression. The goal of RNA-seq experiment was to determine AdpA regulonsduring growth in liquid medium. Dataset contains two strains (adpA deletion mutants and wild type), two time points (12 and 20h).","dates":{"release":"2026-05-01T00:00:00Z","modification":"2026-05-01T01:03:24.918Z","creation":"2025-07-03T17:25:15.72Z"},"accession":"E-MTAB-15315","cross_references":{"ENA":["ERP174615"],"EFO":["EFO_0002944","EFO_0004170","EFO_0004917","EFO_0005518","EFO_0003738","EFO_0004184"]}}