biostudies-arrayexpressKashyap ChhatbarMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15318Setd5 and Ankrd11 are components of the mammalian SET3C complex which are mutated in neuro developmental disorders. We have generated independent clones of mESCs with a missense mutation equivalent to the KBG syndrome mutation E2522K in Ankrd11. We have also generated independent clones of mESCs with W834C and ΔKWK (where K833 to K835 are missing) in Setd5.biostudies-arrayexpressLibrary Construction - DNA was removed using the TURBO DNAfree kit (Ambion) and RNA integrity was assessed on a bioanalyzer. 4µg RNA were used and ERCC Spike-ins were added to prepare sequencing libraries using the ScriptSeq Complete Gold Kit (Illumina). ScriptSeq Index Primers (Illumina) were used to multiplex libraries.Sequencing - Samples were sequenced on an Illumina sequencer at Novogene.Sample Collection - The cells used are mouse embryonic stem cells.Nucleic Acid Extraction - For RNAseq ESC lines were grown for 7-10d to obtain a healthy, fast growing culture. Then 3x105 cells were seeded to 6wells and cells were grown for 48h before isolating RNA using the AllPrep DNA/RNA kit (Qiagen). RNA was prepared from three independent cell seedings to obtain triplicate samples.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Differential gene expression analysis was performed using DESeq2.Sequence Alignment - Annotation data was downloaded from Gencode and STAR aligner and Bowtie2 was used to align reads to the transcriptome and genome respectively.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAMus musculusJenna HareKashyap ChhatbarAdrian BirdKatie PatonMatthew LystfalseRNA-Seq of SETD5 W834C and ANKRD11 E2522K homozygous mESCs with matched controlsSetd5 and Ankrd11 are components of the mammalian SET3C complex which are mutated in neuro developmental disorders. We have generated independent clones of mESCs with a missense mutation equivalent to the KBG syndrome mutation E2522K in Ankrd11. We have also generated independent clones of mESCs with W834C and ΔKWK (where K833 to K835 are missing) in Setd5.2026-04-27T00:00:00Z2026-04-27T11:45:15.15Z2025-07-03T08:22:54.505ZE-MTAB-15318ERP174496E-MTAB-16803EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184