{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Juliette Guitard"],"organism":["Aspergillus fumigatus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15319"],"description":["The aim was to evaluate the transcriptomic pathway impacted by an ETD151 or amphotericine B treatment at 0, 30 minutes, 1 hour, 6 hours.  10^6 conidies of A. fumigatus were incubated in 10 ml YPD for 15 hours. ETD151 (0.156µM) or amphotericin B (1.1µM) was added in the media. After 0, 30 min, 1 hour or 6 hours, RNA from the fungal balls were then extracted using mechanical lysis followed by Nucleospin RNA kit (Macherey Nagel). RNA seq was performed using Illumina NextSeq 500 sequencer."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Aspergillus fumigatus Af293,in vitro culture (YPD medium)","Nucleic Acid Extraction - mechanical lysis ( Magna lysis tubes) : 3 rounds of 3 min at 50 oscillations/min with 10 min of freezing at -80°c between each rounds, repeated twice. Nucleospin miRNA kit (Macherey Nagel®)","Sequencing - Nucleic acid sequencing was realized with Illumina NextSeq 500 sequencer","Sample Treatment - 0, 30 min, 1 hour, 6 hours of either amphotericin B 1.1 µM or ETD151 0.156 µM","Library Construction - Librairies were prepared using the Truseq Stranded mRNA Prep protocol from Illumina, according to manufacturer's instructions, starting from 225ng of total RNA.","Growth Protocol - A; fumigatus was incubated for 15 hours at 37°C under agitation at 100 rpm"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - We used the standard DESeq2 normalization method (DESeq2’s median of ratios with the DESeq function), with a pre-filter of reads and genes (reads uniquely mapped on the genome, or up to 10 different loci with a count adjustment, and genes with at least 10 reads in at least 3 different samples).","Sequence Alignment - Fastq files were aligned using STAR algorithm (version 2.7.6a) on the Ensembl release 101 reference."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["DESeq2","NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"species":["Aspergillus fumigatus"],"pubmed_authors":["Juliette Guitard"],"additional_accession":[]},"is_claimable":false,"name":"transcriptomic analysis of A. fumigatus without or with ETD151 (0.156 µM) or amphotercin B (1.1µM)","description":"The aim was to evaluate the transcriptomic pathway impacted by an ETD151 or amphotericine B treatment at 0, 30 minutes, 1 hour, 6 hours.  10^6 conidies of A. fumigatus were incubated in 10 ml YPD for 15 hours. ETD151 (0.156µM) or amphotericin B (1.1µM) was added in the media. After 0, 30 min, 1 hour or 6 hours, RNA from the fungal balls were then extracted using mechanical lysis followed by Nucleospin RNA kit (Macherey Nagel). RNA seq was performed using Illumina NextSeq 500 sequencer.","dates":{"release":"2026-01-14T00:00:00Z","modification":"2026-01-14T02:02:29.759Z","creation":"2025-07-04T12:15:43.584Z"},"accession":"E-MTAB-15319","cross_references":{"ENA":["ERP174656"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184","EFO_0003969"]}}