biostudies-arrayexpressJuliette GuitardAspergillus fumigatushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15319The aim was to evaluate the transcriptomic pathway impacted by an ETD151 or amphotericine B treatment at 0, 30 minutes, 1 hour, 6 hours. 10^6 conidies of A. fumigatus were incubated in 10 ml YPD for 15 hours. ETD151 (0.156µM) or amphotericin B (1.1µM) was added in the media. After 0, 30 min, 1 hour or 6 hours, RNA from the fungal balls were then extracted using mechanical lysis followed by Nucleospin RNA kit (Macherey Nagel). RNA seq was performed using Illumina NextSeq 500 sequencer.biostudies-arrayexpressSample Collection - Aspergillus fumigatus Af293,in vitro culture (YPD medium)Nucleic Acid Extraction - mechanical lysis ( Magna lysis tubes) : 3 rounds of 3 min at 50 oscillations/min with 10 min of freezing at -80°c between each rounds, repeated twice. Nucleospin miRNA kit (Macherey Nagel®)Sequencing - Nucleic acid sequencing was realized with Illumina NextSeq 500 sequencerSample Treatment - 0, 30 min, 1 hour, 6 hours of either amphotericin B 1.1 µM or ETD151 0.156 µMLibrary Construction - Librairies were prepared using the Truseq Stranded mRNA Prep protocol from Illumina, according to manufacturer's instructions, starting from 225ng of total RNA.Growth Protocol - A; fumigatus was incubated for 15 hours at 37°C under agitation at 100 rpmOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - We used the standard DESeq2 normalization method (DESeq2’s median of ratios with the DESeq function), with a pre-filter of reads and genes (reads uniquely mapped on the genome, or up to 10 different loci with a count adjustment, and genes with at least 10 reads in at least 3 different samples).Sequence Alignment - Fastq files were aligned using STAR algorithm (version 2.7.6a) on the Ensembl release 101 reference.UnknownTranscriptomicsGenomicsProteomicsDESeq2NextSeq 500RNA-seq of coding RNAAspergillus fumigatusJuliette Guitardfalsetranscriptomic analysis of A. fumigatus without or with ETD151 (0.156 µM) or amphotercin B (1.1µM)The aim was to evaluate the transcriptomic pathway impacted by an ETD151 or amphotericine B treatment at 0, 30 minutes, 1 hour, 6 hours. 10^6 conidies of A. fumigatus were incubated in 10 ml YPD for 15 hours. ETD151 (0.156µM) or amphotericin B (1.1µM) was added in the media. After 0, 30 min, 1 hour or 6 hours, RNA from the fungal balls were then extracted using mechanical lysis followed by Nucleospin RNA kit (Macherey Nagel). RNA seq was performed using Illumina NextSeq 500 sequencer.2026-01-14T00:00:00Z2026-01-14T02:02:29.759Z2025-07-04T12:15:43.584ZE-MTAB-15319ERP174656EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969