biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsTim ReganCentrifuge, RNeasy Kit (Qiagen)Scalpel, dry ice.Illumina NovaSeq 6000Genewiz library construction hardwareRNA-seq of total RNAMytilus edulisMytilus edulishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15323Anthropogenic marine noise is increasing around the world and is known to have detrimental effects on many taxa from cetaceans, right down to invertebrates, including marine mussels. Here, we wished to examine the transcriptional changes induced following a week of underwater noise exposure in the gill tissue of blue mussels, and the subsequent response to opportunistic pathogen Vibrio splendidus in control versus noise-exposed animals.biostudies-arrayexpressSequencing - Strand-specific RNA sequencing was performed on the NovaSeq XPLUS platform (Illumina PE150) at 30M reads per sample.Nucleic Acid Extraction - RNA was extracted from gill tissue using the RNeasy Blood & Tissue Kit (Qiagen) according to manufacturer’s instructions.Library Construction - Illumina, 2x150bp, strand specific and Poly-A selection. Performed by Genewiz from Azenta Life Sciences following manufacturer's protocol for Illumina sequencing.Sample Collection - Gill tissue was dissected from mussels and immediately placed on dry ice following either control conditions or 7 days of noise exposure with 48 hours recovery after the noise was turned off. Mussels within each of these groups were also subjected to either control conditions (uninfected) or subjected to 24 hours 1E7 cfu/ml Vibrio splendidus bath challenge for 24 hours.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesTim ReganfalseMytilus edulis Vibrio bacterial challenge following noise exposureAnthropogenic marine noise is increasing around the world and is known to have detrimental effects on many taxa from cetaceans, right down to invertebrates, including marine mussels. Here, we wished to examine the transcriptional changes induced following a week of underwater noise exposure in the gill tissue of blue mussels, and the subsequent response to opportunistic pathogen Vibrio splendidus in control versus noise-exposed animals.2025-08-04T00:00:00Z2025-08-04T10:13:58.031Z2025-07-04T13:04:56.223ZE-MTAB-15323ERP174660E-MTAB-15321EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184