biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsWaradon SungnakNAIllumina HiSeq X10x Chromium™ ControllerRNA-seq of coding RNA from single cellsHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15328This dataset comprises single-cell RNA sequencing (scRNA-seq) and immune receptor (TCR/BCR) profiling of peripheral blood mononuclear cells (PBMCs) collected from individuals infected with dengue virus (DENV) exhibiting different clinical severities: asymptomatic dengue (AD), dengue fever (DF), and dengue hemorrhagic fever (DHF). PBMCs were collected during the acute phase and, in some cases, during convalescence. The data provide a resource for understanding protective versus pathogenic immune responses in dengue infection.biostudies-arrayexpressSequencing - Chromium™ Single Cell 5′ Gene Expression and V(D)J libraries were sequenced using Illumina platforms. Gene expression libraries were sequenced with 26 bp (Read 1) and 98 bp (Read 2)Sample Collection - Frozen peripheral blood mononuclear cells (PBMCs) were thawed at 37°C and diluted five times 1:1 in RPMI containing 10% FBS, following the 10x Genomics protocol for fresh frozen human PBMCs. Dead cells were removed using a Dead Cell Removal Kit (Miltenyi Biotec), and cell clumps were filtered with 40 µm Flowmi® strainers (SP Industries). Cell counts were determined microscopically prior to single-cell capture.Nucleic Acid Extraction - No separate RNA extraction was performed. Single-cell suspensions were directly loaded into the 10x Chromium™ Controller for gel bead-in-emulsion (GEM) generation and reverse transcription.Library Construction - Reverse transcription, cDNA amplification, and library construction were performed using Chromium™ Single Cell V(D)J Reagent Kits with V1 Chemistry (10x Genomics), following the manufacturer’s protocol. Target recovery was 10,000 cells per sample without sample multiplexing.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesWaradon SungnakfalseSingle-cell Transcriptomic and Immune Receptor Profiling of PBMCs from Dengue-Infected Individuals with Varying Disease SeveritiesThis dataset comprises single-cell RNA sequencing (scRNA-seq) and immune receptor (TCR/BCR) profiling of peripheral blood mononuclear cells (PBMCs) collected from individuals infected with dengue virus (DENV) exhibiting different clinical severities: asymptomatic dengue (AD), dengue fever (DF), and dengue hemorrhagic fever (DHF). PBMCs were collected during the acute phase and, in some cases, during convalescence. The data provide a resource for understanding protective versus pathogenic immune responses in dengue infection.2025-08-30T00:00:00Z2025-08-30T01:01:51.778Z2025-07-21T11:14:50.688ZE-MTAB-15328ERP176988EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184