biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMarek MarzecIllumina NovaSeq 6000RNA-seq of coding RNAArabidopsis thalianaArabidopsis thalianahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15333Arabidopsis thaliana plants (WT, SL biosynthesis mutant - max3) were mechanically wounded, Transcriptome response to wounding was analysed after 3 hbiostudies-arrayexpressSequencing - Preparation of RNA library was conducted by Novogene Co., LTD (Beijing, China).Sample Collection - For RNA-isolation analyses, plant tissue (shoot or root) was collected in four biological replicates, each containing tissue from 4 seedlings.Nucleic Acid Extraction - Samples were frozen immediately in liquid nitrogen; RNA was isolated using the mirVana miRNA Isolation Kit (Sigma, catalogue number: AM1560).Growth Protocol - For the RNAseq experiment, plants were grown on Perti dish with 1/2 MS up to 28 days. 12 plants were placed on the single Petri dish. Plants were placed in the greenhouse under a 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity.Library Construction - Preparation of RNA library was conducted by Novogene Co., LTD (Beijing, China).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMarek MarzecfalseRole of strigolactones in response to wounding in ArabidopsisArabidopsis thaliana plants (WT, SL biosynthesis mutant - max3) were mechanically wounded, Transcriptome response to wounding was analysed after 3 h2026-03-02T00:00:00Z2026-03-02T02:02:03.846Z2025-07-08T23:39:08.95ZE-MTAB-15333ERP175057EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184