{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Rocio Perez-Gonzalez"],"study_type":["transcription profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15347"],"description":["In this work, we wanted to investigate the immunological impact of motor fluctuations in Parkinson's disease patients by analyzing blood transcriptomic signatures during motor state transitions.This within-subject design allowed us to isolate dynamic gene expression changes linked specifically to motor state that we could relate with immunological changes."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Blood samples were collected from each study participant into PAXgene Blood RNA tubes according to the manufacturer's protocol (PreAnalytiX GmbH/QIAGEN, Switzerland/US), and stored at -80ºC until RNA analysis","Labeling - Amplified and biotinylated sense-strand cDNA (bssDNA), reverse from total RNA was obtained with the GeneChip WT Plus Reagent kit (Affymetrix Inc., ThermoFisher Scientific)","Nucleic Acid Extraction - Blood Total RNA was isolated using the PAXgene Blood RNA kit (PreAnalytiX GmbH/QIAGEN, Switzerland/US)","Scaning - Samples were scanned with GeneChip Scanner 7G (Affymetrix Inc., ThermoFisher Scientific).","Hybridization - bssDNA samples were hybridized on Clariom D microarrays in a hybridization oven 645 at 45ºC, during 16h, washed and stained in an Affymetrix Fluidics Station."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Rocio Perez-Gonzalez"],"additional_accession":[]},"is_claimable":false,"name":"Whole blood transcriptional profiling of 15 fluctuating Parkinson's disease patients at two time-points: prior to medication (OFF), and following dopaminergic treatment (ON)","description":"In this work, we wanted to investigate the immunological impact of motor fluctuations in Parkinson's disease patients by analyzing blood transcriptomic signatures during motor state transitions.This within-subject design allowed us to isolate dynamic gene expression changes linked specifically to motor state that we could relate with immunological changes.","dates":{"release":"2026-02-02T00:00:00Z","modification":"2026-02-02T02:01:40.951Z","creation":"2025-07-09T15:09:30.44Z"},"accession":"E-MTAB-15347","cross_references":{"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0005518","EFO_0003815"]}}