biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsRocio Perez-Gonzaleztranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15347In this work, we wanted to investigate the immunological impact of motor fluctuations in Parkinson's disease patients by analyzing blood transcriptomic signatures during motor state transitions.This within-subject design allowed us to isolate dynamic gene expression changes linked specifically to motor state that we could relate with immunological changes.biostudies-arrayexpressSample Collection - Blood samples were collected from each study participant into PAXgene Blood RNA tubes according to the manufacturer's protocol (PreAnalytiX GmbH/QIAGEN, Switzerland/US), and stored at -80ºC until RNA analysisLabeling - Amplified and biotinylated sense-strand cDNA (bssDNA), reverse from total RNA was obtained with the GeneChip WT Plus Reagent kit (Affymetrix Inc., ThermoFisher Scientific)Nucleic Acid Extraction - Blood Total RNA was isolated using the PAXgene Blood RNA kit (PreAnalytiX GmbH/QIAGEN, Switzerland/US)Scaning - Samples were scanned with GeneChip Scanner 7G (Affymetrix Inc., ThermoFisher Scientific).Hybridization - bssDNA samples were hybridized on Clariom D microarrays in a hybridization oven 645 at 45ºC, during 16h, washed and stained in an Affymetrix Fluidics Station.MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsRocio Perez-GonzalezfalseWhole blood transcriptional profiling of 15 fluctuating Parkinson's disease patients at two time-points: prior to medication (OFF), and following dopaminergic treatment (ON)In this work, we wanted to investigate the immunological impact of motor fluctuations in Parkinson's disease patients by analyzing blood transcriptomic signatures during motor state transitions.This within-subject design allowed us to isolate dynamic gene expression changes linked specifically to motor state that we could relate with immunological changes.2026-02-02T00:00:00Z2026-02-02T02:01:40.951Z2025-07-09T15:09:30.44ZE-MTAB-15347EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003815