{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Rachael Duodu"],"organism":["Pseudomonas aeruginosa UCBPP-PA14"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15348"],"description":["Comparison of transcriptomes of TamA, TamB and TamAB knock-out strains."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Library preparation (including mRNA depletion, cDNA synthesis, barcoding and pooling of samples) was done by Novogene using their proprietary protocols and reagents.","Nucleic Acid Extraction - RNA was extracted from cell pellets using the Monarch Total RNA Miniprep Kit, according to the manufacturer's instructions with minor modifications (concentration of lysozyme changed to 0.01 mg/ml).","Sequencing - The library was sequenced on a NovaSeq X Plus Series (PE150) machine, generated at least 3Gbp of sequence data per sample.","Sample Collection - Samples were pelleted and RNA extracted immediately."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["RNA-seq of coding RNA"],"species":["Pseudomonas aeruginosa UCBPP-PA14"],"pubmed_title":["The Omp85 family protein, TamA, exhibits characteristics of a suitable drug target against Pseudomonas aeruginosa"],"pubmed_authors":["Jack Leo","Rachael Duodu","Rachael Duodu, David Boocock, Lesley Hoyles, Jack C. Leo"],"additional_accession":[]},"is_claimable":false,"name":"Comparison of transcriptomes of TamA, TamB and TamAB knock-out strains","description":"Comparison of transcriptomes of TamA, TamB and TamAB knock-out strains.","dates":{"release":"2025-09-26T00:00:00Z","modification":"2025-09-26T01:04:50.92Z","creation":"2025-07-09T15:15:36.244Z"},"accession":"E-MTAB-15348","cross_references":{"ENA":["ERP175151"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}