biostudies-arrayexpressRachael DuoduPseudomonas aeruginosa UCBPP-PA14https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15348Comparison of transcriptomes of TamA, TamB and TamAB knock-out strains.biostudies-arrayexpressLibrary Construction - Library preparation (including mRNA depletion, cDNA synthesis, barcoding and pooling of samples) was done by Novogene using their proprietary protocols and reagents.Nucleic Acid Extraction - RNA was extracted from cell pellets using the Monarch Total RNA Miniprep Kit, according to the manufacturer's instructions with minor modifications (concentration of lysozyme changed to 0.01 mg/ml).Sequencing - The library was sequenced on a NovaSeq X Plus Series (PE150) machine, generated at least 3Gbp of sequence data per sample.Sample Collection - Samples were pelleted and RNA extracted immediately.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAPseudomonas aeruginosa UCBPP-PA14The Omp85 family protein, TamA, exhibits characteristics of a suitable drug target against Pseudomonas aeruginosaJack LeoRachael DuoduRachael Duodu, David Boocock, Lesley Hoyles, Jack C. LeofalseComparison of transcriptomes of TamA, TamB and TamAB knock-out strainsComparison of transcriptomes of TamA, TamB and TamAB knock-out strains.2025-09-26T00:00:00Z2025-09-26T01:04:50.92Z2025-07-09T15:15:36.244ZE-MTAB-15348ERP175151EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184