{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Rina Sakata"],"instrument_platform":["NextSeq 2000"],"study_type":["RNA-seq of coding RNA from single cells"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15360"],"description":["scRNAseq of human embryo derived extra-embryonic mesoderm-like cells grown in 2D and 3D"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - library extracted with standard 10x 5' (v3) library construction protocol","Sample Collection - cells were resuspended in dissociation solution containing DMEM/F12 (21331-020,  Thermo Fisher Scientific), 1 mg/mL collagenase I (C2674, Sigma-Aldrich), 1 mg/mL collagenase IV (17104019, Gibco) and 20 U/mL DNase I (M0303S, New England Biolabs) and incubated for 15 min at 37 ºC. Cells were washed with DMEM/F12 containing 0.1% BSA.  Cells were filtered using a 40 µm cell strainer and counted.","Sequencing - sequenced using NextSeq 2000","Library Construction - library was constructed with standard 10x 5' (v3) library construction protocol"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Rina Sakata"],"additional_accession":[]},"is_claimable":false,"name":"scRNAseq of human embryo derived extra-embryonic mesoderm-like cells","description":"scRNAseq of human embryo derived extra-embryonic mesoderm-like cells grown in 2D and 3D","dates":{"release":"2026-08-08T00:00:00Z","modification":"2026-08-08T01:01:01.291Z","creation":"2025-07-14T13:41:37.818Z"},"accession":"E-MTAB-15360","cross_references":{"ENA":["ERP176758"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0004184"]}}