biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsRina SakataNextSeq 2000RNA-seq of coding RNA from single cellsHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15360scRNAseq of human embryo derived extra-embryonic mesoderm-like cells grown in 2D and 3Dbiostudies-arrayexpressNucleic Acid Extraction - library extracted with standard 10x 5' (v3) library construction protocolSample Collection - cells were resuspended in dissociation solution containing DMEM/F12 (21331-020, Thermo Fisher Scientific), 1 mg/mL collagenase I (C2674, Sigma-Aldrich), 1 mg/mL collagenase IV (17104019, Gibco) and 20 U/mL DNase I (M0303S, New England Biolabs) and incubated for 15 min at 37 ºC. Cells were washed with DMEM/F12 containing 0.1% BSA. Cells were filtered using a 40 µm cell strainer and counted.Sequencing - sequenced using NextSeq 2000Library Construction - library was constructed with standard 10x 5' (v3) library construction protocolOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesRina SakatafalsescRNAseq of human embryo derived extra-embryonic mesoderm-like cellsscRNAseq of human embryo derived extra-embryonic mesoderm-like cells grown in 2D and 3D2026-08-08T00:00:00Z2026-08-08T01:01:01.291Z2025-07-14T13:41:37.818ZE-MTAB-15360ERP176758EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184