{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Eugene Berezikov"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15361"],"description":["To investigate the effects of abemaciclib (a CDK4/6 inhibitor) on the secretory phenotype (SASP) and other characteristics of chemotherapy-induced senescent cells, we performed single-cell RNA sequencing on vehicle-treated proliferating BJ fibroblasts, doxorubicin-induced senescent cells, and doxorubicin-induced senescent cells treated with abemaciclib. This approach also enables the resolution of cellular heterogeneity among senescent cells induced by the same stimulus—a level of detail that cannot be captured with bulk RNA sequencing."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - For each sample, 5000 cells were used for the library preparation, aiming to capture approximately 2500 cells.","Nucleic Acid Extraction - Single cells were captured using SeekOne® Digital Droplet System (Beijing SeekGene BioSciences Co., Ltd) according to the manufacturer protocol.","Sample Treatment - 3 groups of BJ fibroblasts (population doubling 32) were treated as below:  1. V+V — Vehicle (water for 24 hours) + Vehicle (water for 2 times 24 hours) at 6 days post 1st treatment.  2. D+V — Doxorubicin (250nM for 24 hours) + Vehicle (water for 2 times 24 hours) at 6 days post 1st treatment.  3. D+A — Doxorubicin (250nM for 24 hours) + Abemaciclib (1uM for 2 times 24 hours) at 6 days post 1st treatment.","Sequencing - The libraries were sequenced on Illumina NextSeq 2000 using P3 v.3 50 -cycle kit, aiming to capture approximately 30 million reads per cell.","Library Construction - Single cell RNA-seq libraries were prepared using SeekOne® DD Single Cell 3’ Transcriptome-seq Kit (Beijing SeekGene BioSciences Co., Ltd) according to the manufacturer protocol."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"data_protocol":["Sequence Alignment - Reads were mapped to human genome (GRCh38.p14) using STARSolo from STAR v.2.7.11a package with the following parameters: --soloCellFilter EmptyDrops_CR --outFilterScoreMin 30 --soloCBmatchWLtype 1MM_multi_Nbase_pseudocounts --soloUMIdedup 1MM_CR --soloType CB_UMI_Simple --soloCBwhitelist P3CB.barcode.txt --soloCBstart 1 --soloCBlen 17 --soloUMIstart 18 --soloUMIlen 12 --soloMultiMappers EM --soloFeatures Gene."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 2000"],"study_type":["RNA-seq of coding RNA from single cells"],"species":["Homo sapiens"],"pubmed_authors":["Kirill Ustyantsev","Eugene Berezikov","Marco Demaria","Boshi Wang"],"additional_accession":[]},"is_claimable":false,"name":"The effects of CDK4/6 inhibitors on chemotherapy-induced senescent cells","description":"To investigate the effects of abemaciclib (a CDK4/6 inhibitor) on the secretory phenotype (SASP) and other characteristics of chemotherapy-induced senescent cells, we performed single-cell RNA sequencing on vehicle-treated proliferating BJ fibroblasts, doxorubicin-induced senescent cells, and doxorubicin-induced senescent cells treated with abemaciclib. This approach also enables the resolution of cellular heterogeneity among senescent cells induced by the same stimulus—a level of detail that cannot be captured with bulk RNA sequencing.","dates":{"release":"2026-06-01T00:00:00Z","modification":"2026-06-01T01:01:17.771Z","creation":"2025-07-14T13:45:04.02Z"},"accession":"E-MTAB-15361","cross_references":{"ENA":["ERP176759"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0004917","EFO_0005518","EFO_0004184","EFO_0003969"]}}