biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsBeatrice BodegaNextSeq 2000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15363In this study, RNA-sequencing was performed on 9 early-onset colorectal cancer (EO-CRC) samples, 8 adjacent normal tissues, and 5 liver metastases. The resulting data were analyzed to characterize gene expression profiling and to explore the activity of transposable elements. This analysis revealed that EO-CRC specifically reactivates placental-like transcriptional features associated with early developmental reprogramming, sustained by the expression of HERVH, a family of retrotransposons involved in the maintenance of pluripotencybiostudies-arrayexpressSample Collection - Tumor, normal adjacent, and metastatic specimens derived from EO-CRC human biopsies were homogenized in Promega Homogenization Buffer.Sequencing - Paired end, 100/120bp reads, Illumina NextSeq 2000.Library Construction - Total RNAseq libraries were prepared using SMARTer Stranded RNA-Seq kit from Takara.Nucleic Acid Extraction - RNA has been extracted with Maxwell RCS simplyRNA Cells kitOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesBeatrice BodegaValeria RanzanifalseRNA-sequencing of Early-Onset Colorectal Cancer (EOCRC) samples, normal adjacent tissue and metastasisIn this study, RNA-sequencing was performed on 9 early-onset colorectal cancer (EO-CRC) samples, 8 adjacent normal tissues, and 5 liver metastases. The resulting data were analyzed to characterize gene expression profiling and to explore the activity of transposable elements. This analysis revealed that EO-CRC specifically reactivates placental-like transcriptional features associated with early developmental reprogramming, sustained by the expression of HERVH, a family of retrotransposons involved in the maintenance of pluripotency2026-09-14T00:00:00Z2026-09-14T14:00:52.669Z2025-07-16T21:16:29.612ZE-MTAB-15363ERP176874EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184