{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Rina Sakata"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA from single cells"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15376"],"description":["scRNAseq of human embryo-derived trophoblast organoids and trophoblast stem cells"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Nucleic extraction was conducted using the Chromium Next GEM Single Cell 5' Reagent Kit v2 (PN-1000263, 10X Genomics) according to the manufacturer's instructions","Sample Collection - Trophoblast organoids were cultured at 37 ºC in 21% O2 and 5% CO2 in TOM. Fresh medium was added every two to three days. When organoids reached a size of ~500 µm they were released from Matrigel domes with Dispase for 20 min at 37 ºC and mechanical disruption by pipetting. Organoids were pelleted at 500 x g for 4 min at 4 ºC, resuspended in TrypLE Express and dissociated for 15 min at 37 ºC. Cells were counted and 10,000 cells were seeded per Matrigel dome on a 24-well plate.  For trophoblast stem cells, when cultures reached ~80% confluency, cells were dissociated with TrypLE for 15 min at 37 ºC, pelleted at 500 x g for 4 min","Library Construction - Nucleic extraction was conducted using the Chromium Next GEM Single Cell 5' Reagent Kit v2 (PN-1000263, 10X Genomics) according to the manufacturer's instructions","Sequencing - Samples were sequenced on a NovaSeq 6000 or NextSeq 2000 at >50,000 reads per cell."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Rina Sakata"],"additional_accession":[]},"is_claimable":false,"name":"scRNAseq of human embryo-derived trophoblast organoids and trophoblast stem cells","description":"scRNAseq of human embryo-derived trophoblast organoids and trophoblast stem cells","dates":{"release":"2026-07-10T00:00:00Z","modification":"2026-07-10T01:00:45.556Z","creation":"2025-07-21T12:38:03.424Z"},"accession":"E-MTAB-15376","cross_references":{"ENA":["ERP176994"],"Biostudies":["E-MTAB-15360"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0004184"]}}