biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsRina SakataIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15376scRNAseq of human embryo-derived trophoblast organoids and trophoblast stem cellsbiostudies-arrayexpressNucleic Acid Extraction - Nucleic extraction was conducted using the Chromium Next GEM Single Cell 5' Reagent Kit v2 (PN-1000263, 10X Genomics) according to the manufacturer's instructionsSample Collection - Trophoblast organoids were cultured at 37 ºC in 21% O2 and 5% CO2 in TOM. Fresh medium was added every two to three days. When organoids reached a size of ~500 µm they were released from Matrigel domes with Dispase for 20 min at 37 ºC and mechanical disruption by pipetting. Organoids were pelleted at 500 x g for 4 min at 4 ºC, resuspended in TrypLE Express and dissociated for 15 min at 37 ºC. Cells were counted and 10,000 cells were seeded per Matrigel dome on a 24-well plate. For trophoblast stem cells, when cultures reached ~80% confluency, cells were dissociated with TrypLE for 15 min at 37 ºC, pelleted at 500 x g for 4 minLibrary Construction - Nucleic extraction was conducted using the Chromium Next GEM Single Cell 5' Reagent Kit v2 (PN-1000263, 10X Genomics) according to the manufacturer's instructionsSequencing - Samples were sequenced on a NovaSeq 6000 or NextSeq 2000 at >50,000 reads per cell.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesRina SakatafalsescRNAseq of human embryo-derived trophoblast organoids and trophoblast stem cellsscRNAseq of human embryo-derived trophoblast organoids and trophoblast stem cells2026-07-10T00:00:00Z2026-07-10T01:00:45.556Z2025-07-21T12:38:03.424ZE-MTAB-15376ERP176994E-MTAB-15360EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184