biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsBen TowlerNextSeq 2000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15388RNAseq of mouse hip cartilage (hip caps) cultured for 44 hours in normoxia (21% O₂) or hypoxia (3% O₂), with or without 100 nM (2.6 µg/ml) recombinant human tissue inhibitor of metalloproteinases-3 (rhuTIMP-3) for the final 20 hours.biostudies-arrayexpressLibrary Construction - The sequencing library was generated with the QuantSeq 3’ mRNA-Seq Library Prep kit FWD for Illumina (Lexogen).Nucleic Acid Extraction - Total RNA was extracted with the miRNeasy kit (QIAGEN) with in-column DNA removal.Sample Collection - Cartilage from mouse hip joints (hip caps) was dissected and cultured (two caps per well in a 96-well plate with serum-free Opti-MEM) under standard conditions for 72 hours. Half the samples were transferred to hypoxia (3% O₂) for 24 hours, after which rhuTIMP-3 was added to subsets of both normoxic and hypoxic samples for 20 hours. Hypoxic samples were cultured for a total of 44 hours in hypoxia. Samples were homogenized in QIAzol with the Precellys lysing kit CK28R and frozen at -80.Sequencing - Sequencing was performed on a NextSeq 2000.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesBen TowlerLisa MullenManuela MengozzifalseGene expression profile in mouse articular cartilage under normoxia (21% O2) and hypoxia (3% O2), with or without TIMP-3RNAseq of mouse hip cartilage (hip caps) cultured for 44 hours in normoxia (21% O₂) or hypoxia (3% O₂), with or without 100 nM (2.6 µg/ml) recombinant human tissue inhibitor of metalloproteinases-3 (rhuTIMP-3) for the final 20 hours.2026-02-12T00:00:00Z2026-02-12T11:16:59.671Z2025-07-22T14:45:11.849ZE-MTAB-15388ERP177064EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184