biostudies-arrayexpressI-Hsuan LinMus musculusCell Ranger v6.1.2https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15391The aim of this experiment was to identify specific subsets of monocytes, dendritic cells, macrophages and structural cells including epithelial cells, endothelial cells and stromal cells in the murine uterus, across all reproductive cycle stages via single cell RNA sequencing. Samples were FACS-enriched separately for populations of CD45+Ly6G-SiglecF-CD3-CD5-CD19-CD20- cells expressing MHC class II and/or CD11b (myeloid) and CD45- structural cells.biostudies-arrayexpressLibrary Construction - Gene expression libraries were prepared from single cells using the Chromium Controller and Single Cell 3ʹ Reagent Kits v3 (10x Genomics, Inc. Pleasanton, USA) according to the manufacturer’s protocol (CG000183 Rev A). Briefly, nanoliter-scale Gel Beads-in-emulsion (GEMs) were generated by combining barcoded Gel Beads, a master mix containing cells, and partitioning oil onto a Chromium chip. Cells were delivered at a limiting dilution, such that the majority (90-99%) of generated GEMs contain no cell, while the remainder largely contain a single cell. The Gel Beads were then dissolved, primers released, and any co-partitioned cells lysed. Primers containing an Illumina TruSeq Read 1 sequencing primer, a 16-nucleotide 10x Barcode, a 12-nucleotide unique molecular ideSequencing - Paired-end sequencing (28:98) was performed on the Illumina NextSeq 500 System using NextSeq 500/550 High Output v2.5 (150 Cycles) reagents at the Genomic Technologies Core Facility at the University of Manchester.Sample Collection - Uterine horns were digested in collagenase V, collagenase D and dispase for 35 minutes prior to antibody staining for FACS enrichment of myeloid cells (CD45+Ly6G-SiglecF-CD3-CD5-CD19-CD20- cells expressing MHC class II and/or CD11b) and CD45- cells.Nucleic Acid Extraction - The cells were passed through the 10x Chromium (10x Genomics) system as indicated on the manufacturer’s protocol (CG000315 Rev B).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSequence Alignment - Raw sequencing data were preprocessed using the 10x Genomics Cell Ranger pipeline v6.1.2 (10x Genomics) following the manufacturer's instructions with default parameters and aligned against the pre-built mouse reference package from 10X Genomics (mm10-2020-A).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 500RNA-seq of coding RNA from single cellsMus musculusElizabeth MannI-Hsuan LinfalseSingle cell RNAseq of uterine myeloid and CD45- cells in the steady stateThe aim of this experiment was to identify specific subsets of monocytes, dendritic cells, macrophages and structural cells including epithelial cells, endothelial cells and stromal cells in the murine uterus, across all reproductive cycle stages via single cell RNA sequencing. Samples were FACS-enriched separately for populations of CD45+Ly6G-SiglecF-CD3-CD5-CD19-CD20- cells expressing MHC class II and/or CD11b (myeloid) and CD45- structural cells.2026-08-07T00:00:00Z2026-08-07T20:07:01.102Z2025-07-24T09:28:49.909ZE-MTAB-15391ERP177199EFO_0002944EFO_0004170EFO_0005684EFO_0004917EFO_0005518EFO_0004184