{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Ian Donaldson"],"organism":["Homo sapiens"],"software":["bcl2fastq v2.20.0.422"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15397"],"description":["The aim of this experiment was to identify differential gene expression pathways in human endometrial FACS-enriched monocytes (CD45+CD66b-Siglec8-CD3-CD19-CD20-HLA-DR+CD64+CD14+CCR2+ cells), Mac1 macrophages (CD45+CD66b-Siglec8-CD3-CD19-CD20-HLA-DR+CD64+CD14+CCR2- CD206- cells), Mac2 macrophages (CD45+CD66b-Siglec8-CD3-CD19-CD20-HLA-DR+CD64+CD14+CCR2- CD206+ cells) and stromal cells (CD45-CD31-EpCAM-Podoplanin+CD90+cells ), in healthy controls and patients who have a prior pregnancy history of pre-eclampsia."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Samples were sequenced on an Illumina NovaSeq6000. Output data was demultiplexed and BCL-to-Fastq conversion performed using Illumina’s bcl2fastq software, version 2.20.0.422.","Sample Collection - Endometrial pipelle biopsies were digested in collagenase 8, collagenase D and dispase for 35 minutes prior to antibody staining for FACS enrichment of monocytes, Mac1 macrophages and Mac2 macrophages.","Library Construction - Library preparation of samples was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kits (Illumina).","Nucleic Acid Extraction - Samples collected in RLT buffer and RNA isolated using Qiagen RNeasy Plus Micro kit."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_authors":["Elizabeth Mann","Ian Donaldson"],"additional_accession":[]},"is_claimable":false,"name":"RNAseq of human endometrial monocytes and macrophages in healthy controls and pre-eclampsia","description":"The aim of this experiment was to identify differential gene expression pathways in human endometrial FACS-enriched monocytes (CD45+CD66b-Siglec8-CD3-CD19-CD20-HLA-DR+CD64+CD14+CCR2+ cells), Mac1 macrophages (CD45+CD66b-Siglec8-CD3-CD19-CD20-HLA-DR+CD64+CD14+CCR2- CD206- cells), Mac2 macrophages (CD45+CD66b-Siglec8-CD3-CD19-CD20-HLA-DR+CD64+CD14+CCR2- CD206+ cells) and stromal cells (CD45-CD31-EpCAM-Podoplanin+CD90+cells ), in healthy controls and patients who have a prior pregnancy history of pre-eclampsia.","dates":{"release":"2026-07-16T00:00:00Z","modification":"2026-07-16T08:46:49.528Z","creation":"2025-07-24T16:24:06.264Z"},"accession":"E-MTAB-15397","cross_references":{"ENA":["ERP177226"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}