{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["thomas cokelaer"],"instrument_platform":["NextSeq 2000"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15406"],"description":["We hypothesized that the transfer of mitochondria via tunneling nanotubes help cancer cells to maintain or boost their metabolism by transferring whole healthy organelles from astrocytes (AS) to tumor cells. To investigate this, we explored the transcriptional changes induced by TNT-dependent transfer from AS to glioblastoma stem cells (+) (GSC). We focused on GSC (+) due to their crucial role in relapse and their reliance on mitochondrial ATP synthesis. We performed bulk RNA sequencing (RNA-seq) on sorted GSC (+) positive for astrocytic-derived mitochondria (mito-dsRED+) and on GSC (+) negative (mito-dsRED-) as controls"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Sequencing was performed on a NextSeq2000 sequencing system (Illumina) to generate 40-60 million reads per sample (150bp).","Library Construction - cDNA libraries were prepared using Illumina Stranded mRNA library Preparation Kit (Illumina) following the manufacturer’s protocol from 250 ng of RNA. Pooled libraries were additionally purified of unbound adaptors and primer dimers on AMPure XP magnetic beads (Beckman-Coulter).","Nucleic Acid Extraction - For the extraction, we use the RNeasy microkit Qiagen","Sample Collection - GSC (+) have received astrocytic mitochondria (mito-dsRED+) and without astrocytic mitochondria (mito-dsRED-) were sorted after 4 days of co-culture in 2D-culture with murine AS expressing mito-dsRED in a ratio of 3 D to 1 Acc (GSC +), to allow the accumulation of mitochondria, from three independent experiments."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Chiara Zurzolo","Ines Saenz-de-Santa-Maria","thomas cokelaer"],"additional_accession":[]},"is_claimable":false,"name":"Tunneling nanotubes mediate mitochondrial homeostasis in cancer","description":"We hypothesized that the transfer of mitochondria via tunneling nanotubes help cancer cells to maintain or boost their metabolism by transferring whole healthy organelles from astrocytes (AS) to tumor cells. To investigate this, we explored the transcriptional changes induced by TNT-dependent transfer from AS to glioblastoma stem cells (+) (GSC). We focused on GSC (+) due to their crucial role in relapse and their reliance on mitochondrial ATP synthesis. We performed bulk RNA sequencing (RNA-seq) on sorted GSC (+) positive for astrocytic-derived mitochondria (mito-dsRED+) and on GSC (+) negative (mito-dsRED-) as controls","dates":{"release":"2025-12-01T00:00:00Z","modification":"2026-03-25T14:26:14.457Z","creation":"2025-07-25T12:45:26.761Z"},"accession":"E-MTAB-15406","cross_references":{"ENA":["ERP177274"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}