{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Jean-Marc Bonneville"],"organism":["Aedes albopictus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15418"],"description":["Gene expression changes were investigated between six mosquito lines originating from La Reunion Island and correlated with their differential deltamethrin resistance levels. Two of these 6 lines derived from a composite population made by pooling six distinct field populations from La Reunion Island: the Sel line was subjected to deltamethrin selection for five successive generations at the adult stage while the NS line was maintained without selection. Three field populations collected in 2018-2029 in three distinct localities (SL: Saint-Louis, SP: Saint Pierre, SS: Sainte-Suzanne) were also used. Finally, the susceptible line SRun originating collected in the early 2000s from La Réunion island and maintained in the laboratory since then was also used as a reference. For each line, four replicate transcriptomes were generated from 5-days old non-blood fed adult females."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - DNB sequencing in multiplex as 100 bp paired-end reads","Sample Collection - Four batches of 25 five days-old non-blood-fed females each processed to extract RNA","Nucleic Acid Extraction - Total RNA was extracted using TRIzol® (ThermoFisher Scientific) according to manufacturer’s instructions. RNA extracts were quantified with a Nanodrop One. Sample quality control was performed on an Agilent 2100.","Library Construction - Libraries were prepared from 10 ng total RNA following the DNB-seq Eukaryotic Strand-specific mRNA library protocol,: mRNAs were enriched in poly A tails using oligodT beads, fragmented to an average size of 200-400 bpc and reverse transcribed using random primers, adaptors were ligated at the 3’ end of the cDNAs, and libraries were amplified by PCR using rolling circle amplification to make DNA nanoballs (DNB)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["DNBSEQ-T7"],"study_type":["RNA-seq of coding RNA"],"species":["Aedes albopictus"],"pubmed_title":["Metabolic resistance of the tiger mosquito to pyrethroid insecticides in La Réunion Island likely results from local adaptation"],"pubmed_authors":["Tiphaine Bacot, Jean-Marc Bonneville, Vincent Lacroix, Pascal Oberbach, Thierry Gaude, Louis Nadalin Frederic Laporte, Nausicaa Habchi-Hanriot, Guillaume Dupuy, Cyrille Czeher, Michael C Fontaine, Frederic Boyer and Jean-Philippe David","Jean-Marc Bonneville"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of 6 Aedes albopictus mosquito lines from La Réunion island with contrasted resistance to the pyrethroid insecticide deltamethrin","description":"Gene expression changes were investigated between six mosquito lines originating from La Reunion Island and correlated with their differential deltamethrin resistance levels. Two of these 6 lines derived from a composite population made by pooling six distinct field populations from La Reunion Island: the Sel line was subjected to deltamethrin selection for five successive generations at the adult stage while the NS line was maintained without selection. Three field populations collected in 2018-2029 in three distinct localities (SL: Saint-Louis, SP: Saint Pierre, SS: Sainte-Suzanne) were also used. Finally, the susceptible line SRun originating collected in the early 2000s from La Réunion island and maintained in the laboratory since then was also used as a reference. For each line, four replicate transcriptomes were generated from 5-days old non-blood fed adult females.","dates":{"release":"2026-06-25T00:00:00Z","modification":"2026-06-25T01:01:03.024Z","creation":"2025-07-28T13:08:27.412Z"},"accession":"E-MTAB-15418","cross_references":{"ENA":["ERP177329"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}