{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Francesco Strozzi"],"instrument_platform":["NA","Illumina NovaSeq X","Chromium 10X Genomics"],"study_type":["RNA-seq of coding RNA from single cells"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15419"],"description":["Single cell RNAseq analysis of in vitro B-cells stimulated with CpG, CGS and Inupadenant"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Tonsil sample collected by adult patients undergoing tonsillectomy was cut into small fragments before mechanical dissociation through a 100 mm cell strainer using a syringe plunger. Tonsillar mononuclear cells (TMNC) were then isolated by lymphoprep density gradient centrifugation using SepMate 50 tubes (STEMCELL Technologies) and tonsillar B cells were enriched using EasySep™ Human Pan-B Cell Enrichment Kit (STEMCELL Technologies) following the manufacturer’s protocol. Isolated B cells were cultured in a 24-well plate at a concentration of 2 million per well in 1ml of X-vivo medium supplemented with 5% inactivated human serum, 1mM of Na-pyruvate and 1mg/ml of CpG for 3h at 37°C, 5% CO2. The A2AR agonist CGS-21680 was added at 5 mM alone or in combination with the A2AR antagonist inupadenant (EOS100850) at 300 nM final concentration.","Library Construction - Library preparation was performed following the Chromium FixedRNAProfiling_MULTIPLEXED Samples (10X Genomics) protocol. Chromium Fixed RNA Kit, Human Transcriptome, 4 rxns x 16 BC, PN-1000476","Sequencing - Sequencing was performed on Illumina NovaSeq, generating 2x150 paired-end reads","Nucleic Acid Extraction - Treated cells were then fixed 1h at room temperature following the Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling (10X Genomics) manufacturer’s protocol."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Paola Tieppo","David Carbonez","Francesco Strozzi"],"additional_accession":[]},"is_claimable":false,"name":"Single cell RNAseq analysis of in vitro B-cells stimulated with CpG, CGS and Inupadenant","description":"Single cell RNAseq analysis of in vitro B-cells stimulated with CpG, CGS and Inupadenant","dates":{"release":"2025-12-07T00:00:00Z","modification":"2025-12-07T02:01:58.36Z","creation":"2025-07-30T15:33:02.987Z"},"accession":"E-MTAB-15419","cross_references":{"ENA":["ERP177492"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0004184"]}}