biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsKathryn Schunketranscription profiling by arrayRattus norvegicusRattus norvegicushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15425We have evidence that Zmynd8 is an epigenetic reader than alters the capability of HIF-1a to bind to its target genes in hypoxia. HIF-1a directly upregulates Zmynd8, thereby working in a negative feedback loop. We aimed to determine if knocking down Zmynd8 in the hypoxic setting would promote HIF-1a mediated transcription.biostudies-arrayexpressScaning - Samples were processed and array scanning was done according to the standard Clariom D sample processing protocol.Nucleic Acid Extraction - RNA was extracted using Qiagen RNeasy Mini kit according to the manufactures recommendations.Labeling - Samples were processed and nucleic acid was labeled according to the standard Clariom D sample processing protocol.Hybridization - Samples were processed and hybridization was performed according to the standard Clariom D sample processing protocol.Sample Collection - 10cm plates were washed with ice cold PBS and processed for mRNA extractionMIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsKathryn SchunkefalseZmynd8 influences the expression of HIF-1α target genesWe have evidence that Zmynd8 is an epigenetic reader than alters the capability of HIF-1a to bind to its target genes in hypoxia. HIF-1a directly upregulates Zmynd8, thereby working in a negative feedback loop. We aimed to determine if knocking down Zmynd8 in the hypoxic setting would promote HIF-1a mediated transcription.2025-08-23T00:00:00Z2025-07-31T22:27:30.397Z2025-07-31T22:27:30.397ZE-MTAB-15425EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003815