{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Marko Vrcan"],"organism":["Ovis aries"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15450"],"description":["Because of the central role of the liver in numerous physiological processes and its possible relation with quality carcass traits in suckling lambs, and considering that female lambs have higher carcass fatness levels, this experiment has analyzed the liver transcriptome of 12 suckling lambs (slaughter age: +/- 25 days), including six males and six females.   RNA was extracted from the liver tissue of the lambs at the time of slaughter following a standard protocol. The RNA integrity value was calculated using the Agilent 2,100 Bioanalyzer equipment (Agilent Technologies, CA, USA). cDNA libraries were sequenced to a minimum depth of 30 million paired-end reads, generating 150 bp stranded paired-end reads using an Illumina Novaseq 6,000 system."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - The extraction of RNA from the liver samples was conducted using a standard protocol for RNA extraction with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. The RNA integrity value was calculated using the Agilent 2,100 Bioanalyzer equipment (Agilent Technologies, CA, USA), which was higher than 7 for all the samples.","Library Construction - The cDNA library construction  was conducted at Novogene in Cambridge (UK) using the UltraTM RNA Library Prep Kit (NEBNext®, MA, USA).","Sequencing - The 12 cDNA libraries were sequenced on an Illumina Novaseq 6,000 system. Each cDNA library was sequenced to a minimum depth of 30 million paired-end reads, generating 150 bp stranded paired-end reads.","Sample Collection - Samples of liver tissue were collected from the carcasses of the 12 lambs under study at the time of slaughter. These samples were preserved in an RNA-stabilizing solution (Ambion RNAlater; Life Technologies) and kept at 4°C for 24 hours. Afterward, the RNA-stabilizing solution was discarded, and the samples were frozen at −80°C until RNA extraction."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000","Trizol reagent (Invitrogen), Agilent 2100 Bioanalyzer","NEBNext UltraTM RNA Library Prep Kit"],"study_type":["RNA-seq of coding RNA"],"species":["Ovis aries"],"pubmed_title":["Elucidating genes and gene networks in liver transcriptome to identify sex-differences in carcass traits between males and females suckling lambs"],"pubmed_authors":["Marko Vrcan","Vrcan, M., Suarez-Vega, A., Fonseca, P.A.S., Alonso-García, M., Arranz, J.J., Gutierrez-Gil, B.","Beatriz Gutierrez-Gil"],"additional_accession":[]},"is_claimable":false,"name":"Liver RNA-Seq data of male and female suckling lambs","description":"Because of the central role of the liver in numerous physiological processes and its possible relation with quality carcass traits in suckling lambs, and considering that female lambs have higher carcass fatness levels, this experiment has analyzed the liver transcriptome of 12 suckling lambs (slaughter age: +/- 25 days), including six males and six females.   RNA was extracted from the liver tissue of the lambs at the time of slaughter following a standard protocol. The RNA integrity value was calculated using the Agilent 2,100 Bioanalyzer equipment (Agilent Technologies, CA, USA). cDNA libraries were sequenced to a minimum depth of 30 million paired-end reads, generating 150 bp stranded paired-end reads using an Illumina Novaseq 6,000 system.","dates":{"release":"2025-12-26T00:00:00Z","modification":"2025-12-26T12:03:23.26Z","creation":"2025-08-04T13:57:00.925Z"},"accession":"E-MTAB-15450","cross_references":{"ENA":["ERP177785"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}