{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Jean-Marc Bonneville"],"organism":["Aedes albopictus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15456"],"description":["Genomic changes associated with resistance to the pyrethroid insecticide deltamethrin were investigated between six mosquito lines all originating from La Reunion Island. Two of these 6 lines, Sel and NS, derived from the same composite population made by pooling six distinct field populations from La Reunion Island: in the lab, the Sel line was subjected to deltamethrin selection for five successive generations at the adult stage while the NS line was maintained without selection. Three lines correspond to field populations collected in 2018-2029 in three distinct localities (SL: Saint-Louis, SP: Saint Pierre, SS: Sainte-Suzanne. Finally, the susceptible line SRun originating collected in the early 2000s from La Réunion island and maintained in the laboratory since then was also used as a reference."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - For each line, a single sequencing library was prepared following the DNBseq plant and animal Whole Genome ReSequencing protocol (https://en.mgi-tech.com/products/). Briefly, DNA was fragmented, size selected (insert size ~350 bp) and end-repaired. Adapters were ligated to the DNA fragments which were then size-selected.","Nucleic Acid Extraction - Genomic DNA was extracted separately for each of the three batches using the GENTRA Puregene kit (QIAGEN) following manufacturer’s instructions, quantified using Qbit (Qiagen) and then pooled in equal DNA quantity.  Sample quality was checked on an Agilent 2100 Bioanalyzer.","Sample Collection - DNA was extracted from 3 pools of 34 females, and pooled to equal amounts","Sequencing - Libraries were amplified by PCR using rolling circle amplification and sequenced in multiplex as 150 bp paired-end reads."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["BGISEQ-500"],"study_type":["DNA-seq"],"species":["Aedes albopictus"],"pubmed_title":["Metabolic resistance of the tiger mosquito to pyrethroid insecticides in La Réunion Island likely results from local adaptation"],"pubmed_authors":["Tiphaine Bacot, Jean-Marc Bonneville, Vincent Lacroix, Pascal Oberbach, Thierry Gaude, Louis Nadalin Frederic Laporte, Nausicaa Habchi-Hanriot, Guillaume Dupuy, Cyrille Czeher, Michael C Fontaine, Frederic Boyer and Jean-Philippe David","Jean-Marc Bonneville"],"additional_accession":[]},"is_claimable":false,"name":"Whole genome pool-Seq of 6 Aedes albopictus mosquito lines from La Reunion island (South-East Indian Ocean) with contrasted resistance levels to the pyrethroid insecticide, deltamethrin","description":"Genomic changes associated with resistance to the pyrethroid insecticide deltamethrin were investigated between six mosquito lines all originating from La Reunion Island. Two of these 6 lines, Sel and NS, derived from the same composite population made by pooling six distinct field populations from La Reunion Island: in the lab, the Sel line was subjected to deltamethrin selection for five successive generations at the adult stage while the NS line was maintained without selection. Three lines correspond to field populations collected in 2018-2029 in three distinct localities (SL: Saint-Louis, SP: Saint Pierre, SS: Sainte-Suzanne. Finally, the susceptible line SRun originating collected in the early 2000s from La Réunion island and maintained in the laboratory since then was also used as a reference.","dates":{"release":"2026-06-26T00:00:00Z","modification":"2026-06-26T01:00:45.501Z","creation":"2025-08-05T21:58:39.78Z"},"accession":"E-MTAB-15456","cross_references":{"ENA":["ERP177858"],"Biostudies":["E-MTAB-15418"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0004184"]}}