biostudies-arrayexpressLloyd SteeleHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15458We generate Visium 10x data from healthy skin (n=2) and inflamed atopic dermatitis skin (n=1). We generate Xenium 10x data from non-lesional atopic dermatitis skin (n=2) and inflamed atopic dermatitis skin (n=1). We use these datasets to locate skin fibroblast subtypes identified from scRNA-seq.biostudies-arrayexpressLibrary Construction - As per 10x genomics manufacture's protocolSequencing - As per 10x genomics manufacture's protocolSample Collection - All research ethics committee and regulatory approval were in place for the collection of research samples at Newcastle and for their storage at the Newcastle Dermatology Biobank (REC reference number: 19/NE/0004)Nucleic Acid Extraction - As per 10x genomics manufacture's protocolOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Standard Space Ranger output for 10x including gene matrices; barcodes etcUnknownTranscriptomicsGenomicsIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsHomo sapiensLloyd SteelefalseSpatial localisation of human skin fibroblastsWe generate Visium 10x data from healthy skin (n=2) and inflamed atopic dermatitis skin (n=1). We generate Xenium 10x data from non-lesional atopic dermatitis skin (n=2) and inflamed atopic dermatitis skin (n=1). We use these datasets to locate skin fibroblast subtypes identified from scRNA-seq.2025-08-01T00:00:00Z2025-08-05T22:13:57.179Z2025-08-05T22:13:57.179ZE-MTAB-15458ERP177859EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184