biostudies-arrayexpressLisa DUGASTHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15460CD8 T cells from blood from four healthy volunteers was analysed for several markers by CITEseq and single cell RNAseq.biostudies-arrayexpressLibrary Construction - Single cell libraries were performed according to Chromium Next GEM Single Cell 5' Reagent Kits v2 protocol (10X genomics, San Francisco, CA).Nucleic Acid Extraction - Disintegration of cells or tissues (cell lysis), inactivation of intracellular nucleases, and separation of nucleic acids from the other cell componentsSequencing - Cell suspension were loaded onto chromium single cell chip K in 4 different well (20 000 cells per well) and run immediately on the Chromium controller (10X genomics, San Francisco, CA). Three libraries were prepared: one for mRNA (RNA), one for barcoded antibodies (HTO and CITE-seq) and one for TCR (VDJ-seq). They were sequenced with NovaSeq 6000 (Illumina, San Diego, CA) according to 10X genomics recommendations.Sample Collection - Freshly sorted CD8+ T lymphocytes of four healthy volunteers were labelled with anti-human Hashtag antibodies TotalSeq-C (Biolegend, San Diego, CA) and were pooled together.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - FASTQ files, first generated from BaseCalling (BCL) files with CellRanger package (6.1.2), were demultiplexed and aligned to human reference genome (hg38). CITE-seq-Count function (1.3.4) was used to count antibody hashtag sequences. Count matrixes were analyzed with Seurat R package (4.1.1). Cells with more than 10% of mitochondrial genes were excluded. Gene expression was log normalized and scaled. HTO expression was normalized and demultiplexed. Doublet cells and negative cells for HTO were removed.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsHomo sapiensLisa DUGASTCarole GUILLONNEAUfalseCD8 T cells single cell transcriptomic analysis from human bloodCD8 T cells from blood from four healthy volunteers was analysed for several markers by CITEseq and single cell RNAseq.2025-09-01T00:00:00Z2025-09-01T08:19:16.083Z2025-08-15T10:50:36.531ZE-MTAB-15460ERP178813EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184