{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Weiran Shan"],"instrument_platform":["Illumina MiSeq"],"study_type":["DNA-seq"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15462"],"description":["Six- to eight-week old CD1 male mice were sleep-deprived for 24 hours by placing them in a small platform in a water tank. Their ileum samples were harvested every 4 hours on the next day (time point: ZT1,ZT5,ZT9,ZT13,ZT17,ZT21). The ileum samples were subjected to 16s rDNA sequencing. Control group (C1,C5,C9,C13,C17,C21, n=36). SD group (SD1,SD5,SD9,SD13,SD17,SD21, n=36)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Mouse fecal bacterial DNA was extracted with Power Lyzer Power soil DNA isolation kit (catalog number: 12855-100, QIAGEN, Germantown, MD, USA)","Sample Collection - Control and Sleep deprivation groups of mouse ileum feces were harvested at time points ZT1,ZT5,ZT9,ZT13,ZT17 and ZT21.","Library Construction - Library was created by primers (341F/805R) designed to target the V3 and V4 regions of 16S rDNA, generate an amplicon about 465 bp in size.","Sequencing - The amplified library is sequenced on a NovaSeq platform with 250 bp paired-end reads mode (2 x 250 bp)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Weiran Shan"],"additional_accession":[]},"is_claimable":false,"name":"Sleep deprivation disturbs diurnal rhythmicity of gut microbiota and inflammatory responses in mice","description":"Six- to eight-week old CD1 male mice were sleep-deprived for 24 hours by placing them in a small platform in a water tank. Their ileum samples were harvested every 4 hours on the next day (time point: ZT1,ZT5,ZT9,ZT13,ZT17,ZT21). The ileum samples were subjected to 16s rDNA sequencing. Control group (C1,C5,C9,C13,C17,C21, n=36). SD group (SD1,SD5,SD9,SD13,SD17,SD21, n=36).","dates":{"release":"2025-08-29T00:00:00Z","modification":"2025-08-29T01:02:58.59Z","creation":"2025-08-08T13:34:50.021Z"},"accession":"E-MTAB-15462","cross_references":{"ENA":["ERP178561"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0004184"]}}