biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsWeiran ShanIllumina MiSeqDNA-seqMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15462Six- to eight-week old CD1 male mice were sleep-deprived for 24 hours by placing them in a small platform in a water tank. Their ileum samples were harvested every 4 hours on the next day (time point: ZT1,ZT5,ZT9,ZT13,ZT17,ZT21). The ileum samples were subjected to 16s rDNA sequencing. Control group (C1,C5,C9,C13,C17,C21, n=36). SD group (SD1,SD5,SD9,SD13,SD17,SD21, n=36).biostudies-arrayexpressNucleic Acid Extraction - Mouse fecal bacterial DNA was extracted with Power Lyzer Power soil DNA isolation kit (catalog number: 12855-100, QIAGEN, Germantown, MD, USA)Sample Collection - Control and Sleep deprivation groups of mouse ileum feces were harvested at time points ZT1,ZT5,ZT9,ZT13,ZT17 and ZT21.Library Construction - Library was created by primers (341F/805R) designed to target the V3 and V4 regions of 16S rDNA, generate an amplicon about 465 bp in size.Sequencing - The amplified library is sequenced on a NovaSeq platform with 250 bp paired-end reads mode (2 x 250 bp).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesWeiran ShanfalseSleep deprivation disturbs diurnal rhythmicity of gut microbiota and inflammatory responses in miceSix- to eight-week old CD1 male mice were sleep-deprived for 24 hours by placing them in a small platform in a water tank. Their ileum samples were harvested every 4 hours on the next day (time point: ZT1,ZT5,ZT9,ZT13,ZT17,ZT21). The ileum samples were subjected to 16s rDNA sequencing. Control group (C1,C5,C9,C13,C17,C21, n=36). SD group (SD1,SD5,SD9,SD13,SD17,SD21, n=36).2025-08-29T00:00:00Z2025-08-29T01:02:58.59Z2025-08-08T13:34:50.021ZE-MTAB-15462ERP178561EFO_0002944EFO_0004170EFO_0002693EFO_0005518EFO_0004184