biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsAndrej BesseIllumina NovaSeq XDNA-seqHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15465AMO-1 cells were transduced with Brunello CRISPR screening library at MOI 0.3, and culture in the presnece of ceritinib. DNA was isolated and one step PCR was perfomed and gRNAs conferring to resistance or sensitivity was analysed using MAGECK-VISPR.biostudies-arrayexpressSample Collection - Cells were harvested and DNA was isolated and stored in -80 degreeSequencing - PCR libraries were sequenced using NovaSeq, PE150 partial lane sequencingLibrary Construction - PCR libraries were prepared according to established protocol and primers published here doi: 10.1158/0008-5472.CAN-20-3323.Nucleic Acid Extraction - DNA was isolated using Qiagen Blood & Cell Culture DNA Kits 500/g cca 50-60-mio cellsOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesAndrej Bessefalsewhole genome CRISPR Screening (Brunello library) on AMO-1 cell line treated with CeritnibAMO-1 cells were transduced with Brunello CRISPR screening library at MOI 0.3, and culture in the presnece of ceritinib. DNA was isolated and one step PCR was perfomed and gRNAs conferring to resistance or sensitivity was analysed using MAGECK-VISPR.2025-12-15T00:00:00Z2025-12-15T02:01:05.282Z2025-08-08T14:03:05.228ZE-MTAB-15465ERP178565E-MTAB-15466EFO_0002944EFO_0004170EFO_0002693EFO_0005518EFO_0004184