biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsAndrej BesseMGISEQ-2000RSRNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15466AMO-1 cell line after 8h of Ceritinib, Carfilzomib treatment and their combination. Cells were continuously treated for 8 h with 4uM Ceritinib and 5nM Carfilzomib. Cell pelets were stored at -80 degree and send on dry ice to BGI company.biostudies-arrayexpressGrowth Protocol - Cells were grown in RPMI medium supplemented with 10% FBS and anitbioticsSample Treatment - Cells were treated with Carfilzomib 5nM or Ceritinib 4uM or combination or without for 8hSample Collection - Cell were harvested form T75 flask and washed 2x with PBS and snap frozen in Liquid nitrogenLibrary Construction - polyadenylated RNA was enriched using oligo(dT) magnetic beads. First- and second-strand cDNA synthesis was performed using reverse transcriptase and random hexamer primers. After end repair, A-tailing, and adaptor ligation, libraries were amplified by PCR. The final libraries were quality-checked using Qubit and Bioanalyzer before sequencing. Library construction followed BGI’s standard mRNA-seq protocolSequencing - Sequencing was performed by BGI using the DNBseq platform, generating paired-end 150 bp reads (PE150). Libraries were circularized, amplified via rolling circle replication to form DNA nanoballs (DNBs), and loaded onto patterned arrays for high-throughput sequencingNucleic Acid Extraction - Total RNA was extracted by BGI from the submitted tissue samples using TRIzol reagent or a similar phenol-based method, following their standard RNA extraction workflow. RNA integrity and quantity were assessed using Bioanalyzer or similar QC platformsOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesAndrej BessefalseAMO-1 cell line after 8h of Ceritinib, Carfilzomib treatment and their combinationAMO-1 cell line after 8h of Ceritinib, Carfilzomib treatment and their combination. Cells were continuously treated for 8 h with 4uM Ceritinib and 5nM Carfilzomib. Cell pelets were stored at -80 degree and send on dry ice to BGI company.2025-12-15T00:00:00Z2025-12-15T02:01:04.983Z2025-08-08T15:19:06.566ZE-MTAB-15466ERP178570E-MTAB-15465EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184EFO_0003969