biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsZheng MinHiSeq X TenRNA-seq of coding RNAEpinephelus fuscoguttatusEpinephelus fuscoguttatushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15472Edwardsiella ictaluri, a significant pathogen in aquaculture, can lead to severe liver damage and metabolic disorders in fish. Verification via Transcriptome Sequencing of the liver damage caused by Edwardsiella ictaluri in Hybrid Groupers.biostudies-arrayexpressLibrary Construction - cDNA libraries were constructed as per the instruction manual of NEBNext Ultra RNA Library Prep Kit.Nucleic Acid Extraction - Total RNA was extracted from the midgut of hybrid groupers by using the TRlzol Reagent and following the manufacturer’s instructions (Life Technologies, California, USA).Sample Collection - Before the start of the experiment, 600 healthy hybrid groupers with an average body weight of 42.3 ± 2.8 g were acclimatized in a recirculating aquaculture system (water temperature: 28–30°C; pH: 7.8 ± 0.2) and fasted for 24 h. The fish were then randomly allocated to two treatment groups: Control group ( con)which received no treatment; E. ictaluri-infected group (ADH) which was administered E. ictaluri via oral gavage.Sequencing - These built cDNA libraries were sequenced on an Illumina Hi-Seq X Ten system by the OE Biotech Co., Ltd. (Shanghai, China).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesZheng MinfalseModulation of liver immune-metabolic pathway in hybrid grouper infected with Edwardsiella ictaluriEdwardsiella ictaluri, a significant pathogen in aquaculture, can lead to severe liver damage and metabolic disorders in fish. Verification via Transcriptome Sequencing of the liver damage caused by Edwardsiella ictaluri in Hybrid Groupers.2025-08-20T00:00:00Z2025-08-08T18:15:53.221Z2025-08-08T18:15:53.221ZE-MTAB-15472ERP178582EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184