{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Eslam Elsayed M."],"instrument_platform":["Illumina NovaSeq X"],"study_type":["DNA-seq"],"organism":["Klebsiella pneumoniae"],"species":["Klebsiella pneumoniae"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15474"],"description":["To evaluate potential off-target effects of the RECKLEEN system, we sequenced the genome of the WT Klebsiella pneumoniae MGH 78578 strain and the Δwzi strain that had been generated using the RECKLEEN system."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - The WT Klebsiella pneumoniae MGH 78578 and the Δwzi strain were plated on LB agar and three clones were picked for overnight cultures.","Nucleic Acid Extraction - Total DNA for resequencing was extracted using a DNeasy Blood and Tissue kit (Qiagen) according to the manual.","Library Construction - Quality control and DNA library were performed by Azenta.","Sequencing - Quality control, DNA library preparation, and sequencing were performed by Azenta using Illumina NovaSeq (2x150 bp sequencing, ∼700Mb paired-end reads)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Eslam Elsayed M."],"additional_accession":[]},"is_claimable":false,"name":"DNA-Seq data of WT Klebsiella pneumoniae MGH 78578 and the Δwzi strain","description":"To evaluate potential off-target effects of the RECKLEEN system, we sequenced the genome of the WT Klebsiella pneumoniae MGH 78578 strain and the Δwzi strain that had been generated using the RECKLEEN system.","dates":{"release":"2025-08-30T00:00:00Z","modification":"2025-08-30T01:01:55.816Z","creation":"2025-08-08T16:21:32.221Z"},"accession":"E-MTAB-15474","cross_references":{"ENA":["ERP178577"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0004184"]}}