biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsEslam Elsayed M.Illumina NovaSeq XDNA-seqKlebsiella pneumoniaeKlebsiella pneumoniaehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15474To evaluate potential off-target effects of the RECKLEEN system, we sequenced the genome of the WT Klebsiella pneumoniae MGH 78578 strain and the Δwzi strain that had been generated using the RECKLEEN system.biostudies-arrayexpressSample Collection - The WT Klebsiella pneumoniae MGH 78578 and the Δwzi strain were plated on LB agar and three clones were picked for overnight cultures.Nucleic Acid Extraction - Total DNA for resequencing was extracted using a DNeasy Blood and Tissue kit (Qiagen) according to the manual.Library Construction - Quality control and DNA library were performed by Azenta.Sequencing - Quality control, DNA library preparation, and sequencing were performed by Azenta using Illumina NovaSeq (2x150 bp sequencing, ∼700Mb paired-end reads).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesEslam Elsayed M.falseDNA-Seq data of WT Klebsiella pneumoniae MGH 78578 and the Δwzi strainTo evaluate potential off-target effects of the RECKLEEN system, we sequenced the genome of the WT Klebsiella pneumoniae MGH 78578 strain and the Δwzi strain that had been generated using the RECKLEEN system.2025-08-30T00:00:00Z2025-08-30T01:01:55.816Z2025-08-08T16:21:32.221ZE-MTAB-15474ERP178577EFO_0002944EFO_0004170EFO_0002693EFO_0005518EFO_0004184