biostudies-arrayexpressMiguel Gonzalez AceraMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15483Whole right brain hemispheres of mice with colitis caused by induced, intestinal epithelial-specific Opa1 knock-out to study the impact of gut inflammation on the brainbiostudies-arrayexpressSequencing - The clustering of the index-coded samples was performed according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generatedSample Collection - On day 24 after first tamoxifen administration, mice were sacrificed, followed by transcardial perfusion and isolation of the right brain hemisphereNucleic Acid Extraction - RNA isolation was performed using the RNeasy Mini kit (QIAGEN) following the manufacturer’s instructions.Sample Treatment - Opa1fl/fl x VillinCreERT2 mice (Bao et al., Sci Immunol (2025)) were treated with 75 mg/kg body weight tamoxifen p.o. for five consecutive days to induce Opa1 deletion specifically from the intestinal epithelium leading to epithelial barrier damage and colitis. VillinCreERT negative littermates treated with tamoxifen served as controls.Library Construction - Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Normalization of the data was performed by DESeq2 using the mean of ratios.Sequence Alignment - Sequence mapping and alignment was performed using STAR v2.7.10bUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusHeidi LimbergerJay PatankarMiguel Gonzalez AcerafalseBulk RNA-Sequencing of brain hemispheres of mice with colitis induced by intestinal epithelial-specific Opa1 deletionWhole right brain hemispheres of mice with colitis caused by induced, intestinal epithelial-specific Opa1 knock-out to study the impact of gut inflammation on the brain2026-01-01T00:00:00Z2026-01-02T02:02:38.222Z2025-08-13T14:34:35.835ZE-MTAB-15483ERP178731E-MTAB-15482E-MTAB-15484E-MTAB-15489E-MTAB-15485E-MTAB-15488EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969