biostudies-arrayexpressMiguel Gonzalez AceraMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15484Whole right brain hemispheres of mice with Citrobacter rodentium infection-induced colitis and controls to study the impact of gut inflammation on the brainbiostudies-arrayexpressSequencing - The clustering of the index-coded samples was performed according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generatedSample Treatment - After 8 hours of fasting, male C57BL/6J mice were infected with 109 colony-forming units of C. rodentium strain ICC169 in PBS by oral gavage. Control mice received 200µL PBS orally.Sample Collection - On day 8 post infection, mice were sacrificed, transcardially perfused with PBS and right brain hemispheres collectedNucleic Acid Extraction - RNA isolation was performed using the RNeasy Mini kit (QIAGEN) following the manufacturer’s instructions.Library Construction - Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Normalization of the data was performed by DESeq2 using the mean of ratios.Sequence Alignment - Sequence mapping and alignment was performed using STAR v2.7.10bUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusHeidi LimbergerJay PatankarMiguel Gonzalez AcerafalseBulk RNA-Sequencing of brain hemispheres of mice with colitis caused by Citrobacter rodentium infectionWhole right brain hemispheres of mice with Citrobacter rodentium infection-induced colitis and controls to study the impact of gut inflammation on the brain2026-01-01T00:00:00Z2026-01-02T02:02:38.181Z2025-08-13T14:37:00.047ZE-MTAB-15484ERP178732E-MTAB-15482E-MTAB-15483E-MTAB-15489E-MTAB-15485E-MTAB-15488EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969