biostudies-arrayexpressMiguel Gonzalez AceraMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15489Selected brain regions of mice with acute DSS colitis and controls to study the impact of gut inflammation on the brainbiostudies-arrayexpressSequencing - The clustering of the index-coded samples was performed according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generatedSample Collection - Male C57BL/6J mice were subjected to 3% DSS dissolved in drinking water for 7 days and sacrificed on day 8Nucleic Acid Extraction - RNA isolation was performed using the RNeasy Mini kit (QIAGEN) following the manufacturer’s instructions.Library Construction - Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Normalization of the data was performed by DESeq2 using the mean of ratios.Sequence Alignment - Sequence mapping and alignment was performed using STAR v2.7.10bUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusHeidi LimbergerJay PatankarMiguel Gonzalez AcerafalseBulk RNA-Sequencing of defined brain regions of mice with acute DSS-induced colitisSelected brain regions of mice with acute DSS colitis and controls to study the impact of gut inflammation on the brain2026-01-01T00:00:00Z2026-01-02T02:02:37.477Z2025-08-13T14:58:44.081ZE-MTAB-15489ERP178737E-MTAB-15482E-MTAB-15484E-MTAB-15483E-MTAB-15485E-MTAB-15488EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184