biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMei Meitranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15495DRD2 antagonists are commonly used clinically for the treatment of psychiatric disorders. This study aimed to explore the antitumor effects of DRD2 antagonists on a variety of tumor entities. A DRD2 antagonist, pimozide, exhibited antitumor effects in pancreatic, colorectal, ovarian, and gastric cancers. HMOX1 was found to be highly expressed at both mRNA and protein levels, corroborating the results of profiling. The treatment of pimozide and overexpression of HMOX1 experiments were conducted then.biostudies-arrayexpressLabeling - The expression profile was carried out at the Microarray Unit of the Genomics and Proteomics Core Facility, German Cancer Research Center (DKFZ)Sample Collection - From cell lines. All cell lines stored in our lab were authenticated and confirmed to be mycoplasma-negative before starting the experiments. Cells transfected with ov-HMOX1 and/or ov-NC, treated with pimozide/DMSO.Scaning - The expression profile was carried out at the Microarray Unit of the Genomics and Proteomics Core Facility, German Cancer Research Center (DKFZ)Nucleic Acid Extraction - The process of obtaining mRNA from cells was performed using the RNeasy Mini Kit (Qiagen, Germany). The concentration was measured using NanoDrop Spectrophotometer ND-1000 (Thermo Fisher Scientific, Waltham, Massachusetts, USA).Hybridization - The expression profile was carried out at the Microarray Unit of the Genomics and Proteomics Core Facility, German Cancer Research Center (DKFZ)MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsMei MeiJörg HoheiselData Transformation - Chipster was used for differential expression analysis. Data were normalized using the Robust Multi-array Average (RMA) normalization method.falseTranscriptional profile of overexpression of HMOX1 or/and pimozide-treated cancer cell lines MIA PaCa-2, HCT 116, SK-OV-3, and AGSDRD2 antagonists are commonly used clinically for the treatment of psychiatric disorders. This study aimed to explore the antitumor effects of DRD2 antagonists on a variety of tumor entities. A DRD2 antagonist, pimozide, exhibited antitumor effects in pancreatic, colorectal, ovarian, and gastric cancers. HMOX1 was found to be highly expressed at both mRNA and protein levels, corroborating the results of profiling. The treatment of pimozide and overexpression of HMOX1 experiments were conducted then.2025-08-30T00:00:00Z2025-08-30T01:01:57.79Z2025-08-14T10:24:40.342ZE-MTAB-15495E-MTAB-15496EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815