biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsRachel ScholeyIllumina HiSeq 4000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15512Photoaggravated atopic dermatitis (PAD) is clinically characterised by sunlight induced/aggravated eczema. The underlying cutaneous pathophysiology of PAD remains poorly understood. This study aimed to investigate cellular and molecular events in the evolution of PAD responses following solar simulated ultraviolet radiation (SSR) exposure and compare with responses in healthy controls (HC). Cutaneous biopsies were taken from unexposed skin and from skin exposed to a single low (physiological) dose of SSR, at 30 minutes, 3 hours and 24 hours post-initial exposure, as well as at 72 hours (after three consecutive daily SSR doses), in n=6 PAD patients and n=6 HC. Biopsies were assessed by immunohistochemistry (n=6 PAD, n=6 HC) and RNA-sequencing analysis (n=5 PAD, n=4 HC). This dataset contains RNAseq data from the PAD patients.biostudies-arrayexpressLibrary Construction - Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.Nucleic Acid Extraction - Total RNA was extracted from bisected 5mm skin punch biopsies at baseline, 30 minutes, 3 hours, 24 hours, and 72 hours post-initial UVR provocation using the RNAeasy Plus Universal Minikit (Qiagen, Manchester, UK) according to manufacturer’s protocol and guidelines.Sample Collection - Cutaneous biopsies were taken from buttock skin, including unexposed skin and from skin exposed to a single low (physiological) dose of SSR, at 30 minutes, 3 hours and 24 hours, as well as 72 hours (after three consecutive daily SSR doses) post-initial exposure, in n=6 PAD patients and n=6 HC.Sequencing - Sequenced paired end 76bp on HISEQ4000 at the Genomic Technologies Core Facility University of Manchester.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesLesley RhodesRachel ScholeyfalseRNAseq of human buttock skin from patients with photoaggravated atopic dermatitis exposed to solar simulated radiationPhotoaggravated atopic dermatitis (PAD) is clinically characterised by sunlight induced/aggravated eczema. The underlying cutaneous pathophysiology of PAD remains poorly understood. This study aimed to investigate cellular and molecular events in the evolution of PAD responses following solar simulated ultraviolet radiation (SSR) exposure and compare with responses in healthy controls (HC). Cutaneous biopsies were taken from unexposed skin and from skin exposed to a single low (physiological) dose of SSR, at 30 minutes, 3 hours and 24 hours post-initial exposure, as well as at 72 hours (after three consecutive daily SSR doses), in n=6 PAD patients and n=6 HC. Biopsies were assessed by immunohistochemistry (n=6 PAD, n=6 HC) and RNA-sequencing analysis (n=5 PAD, n=4 HC). This dataset contains RNAseq data from the PAD patients.2026-09-21T00:00:00Z2026-09-21T12:20:46.873Z2025-08-28T09:32:06.08ZE-MTAB-15512ERP179250E-MTAB-12218EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184